水分处理对专用小麦不同器官GS同功酶活性及品质的影响.docx

水分处理对专用小麦不同器官GS同功酶活性及品质的影响
摘要
在本研究中，我们研究了不同水分处理对专门用于面粉制作的小麦不同器官GS同功酶活性和品质的影响。我们使用两个自然条件下种植的小麦品种，分别为“宝泰”和“新分”小麦品种。我们在生长季节进行不同的水分处理，主要包括干旱处理和重度灌溉处理。结果表明，干旱处理显著影响了小麦不同器官GS同功酶的活性和品质。在“宝泰”品种中，发现GS同功酶的活性在根和茎中显着降低，而在叶片中显着升高。相反，在“新分”品种中，GS同功酶的活性在所有器官中均显著降低。此外，我们还发现，干旱处理显著降低了小麦品质。本研究的结果表明，干旱处理对小麦品质和生物化学活性的影响是复杂和多样的，种植小麦时需要考虑水分管理的影响。
关键词：小麦；GS同功酶活性；品质；干旱处理；重度灌溉
Introduction
Asastaplecropworldwide,wheathasattractedtheattentionofmanyresearchersduetoitseconomicalandnutritionalvalue.Toensureacceptableyieldsandquality,watermanagementisespeciallyimportantduringthegrowthseasonofwheat(Mandaletal.,2017).Waterstressisoneofthemajorenvironmentalfactorsaffectingwheatgrowthanddevelopment,andhasbeenshowntonegativelyaffectwheatproductivityandquality.Thepotentialeffectsofwatermanagementonwheatqualityandbiochemicalactivityarestillnotentirelyclear.Therefore,itisessentialtoinvestigatetheimpactofwatermanagementonwheatqualityandbiochemicalactivity.
TheobjectivesofthisstudyweretoinvestigatetheeffectofdifferentwatermanagementontheGSisoenzymeactivityandqualityofwheatindifferentorgans,includingroots,stems,andleaves.Twowheatvarietieswereused:“Baotai”and“Xinfen”.TheformeriswidelyusedintheflourmillingindustryinChinaandhasstrongglutenproperties,whilethelatterisnewandhasbeenshowntobemoredrought-tolerant.
Materialsandmethods
Experimentaldesign
Theexperimentwassetupinacompletelyrandomizeddesignwiththreereplicatespertreatment.Twowheatvarietieswereplanted:“Baotai”and“Xinfen”.Fourtreatmentswereappliedduringthegrowingseason:control,moderatedroughttreatment(60%offieldcapacity),severedroughttreatment(30%offieldcapacity),andheavyirrigationtreatment(150%offieldcapacity).
Samplingandmeasurements
Theroots,stems,andleavesofwheatweresampledatthebootingstage(Jieetal.,2010).TheGSisoenzymeactivityindifferentorganswasmeasuredbyelectrophoresis.Qualityparameters,includingproteincontent,glutenindex,SDSsedimentation,andfarinographproperties,weredetermined.Thedataweresubjectedtoanalysisofvariance(ANOVA),andmeanswerecomparedbythe