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抗人CD133单克隆抗体的制备及CD133抗原在肝癌组织中的表达.docx

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抗人CD133单克隆抗体的制备及CD133抗原在肝癌组织中的表达 Abstract CD133isaglycoproteinthatisexpressedonthesurfaceofvariousstemcells.Ithasbeenshowntobeamarkerfortheidentificationandisolationofcancerstemcellsinseveraltypesofmalignancies,includinglivercancer.Inthisstudy,wedevelopedamonoclonalantibodyagainstCD133andanalyzedtheexpressionofCD133antigeninlivercancertissue.TheresultsshowedthatourmonoclonalantibodyspecificallyrecognizedtheCD133antigenandthattheexpressionofCD133wassignificantlyhigherinlivercancertissuecomparedtoadjacentnormaltissue.ThesefindingssuggestthatCD133maybeapotentialtherapeutictargetforlivercancer. Introduction Livercancer,alsoknownashepatocellularcarcinoma(HCC),isoneofthemostcommonmalignanciesintheworld.Itisthefifthmostcommoncancerandthethirdmostcommoncauseofcancer-relateddeathsglobally[1].Despitesignificantadvancesindiagnosisandtreatment,theprognosisforlivercancerpatientsremainspoor,witha5-yearsurvivalrateoflessthan20%[2].Oneofthereasonsforthepoorprognosisisthepresenceofcancerstemcells(CSCs)inlivercancer.CSCsareasmallpopulationofcellswithinatumorthathasstemcell-likepropertiesandisresponsiblefortumorinitiation,propagation,andresistancetotherapy[3]. CD133,alsoknownasprominin-1,isaglycoproteinthatisexpressedonthesurfaceofvariousstemcells,includinghematopoietic,neural,andepithelialstemcells[4].Inrecentyears,CD133hasbeenidentifiedasamarkerfortheidentificationandisolationofCSCsinseveraltypesofmalignancies,includinglivercancer[5].SeveralstudieshaveshownthatCD133+cellsinlivercancerarehighlytumorigenicandhaveahigherabilitytoself-renewanddifferentiateintodifferentcelltypes[6].Additionally,theexpressionofCD133hasbeenassociatedwithpoorprognosisinlivercancer[7]. Monoclonalantibodiesarehighlyspecificandsensitivetoolsforthedetectionandcharacterizationofproteins.Inthisstudy,wedevelopedamonoclonalantibodyagainstCD133andanalyzedtheexpressionofCD133antigeninlivercancertissue. MaterialsandMethods Celllines ThehumanlivercancercelllineHepG2wasobtainedfromtheAmericanTypeCultureCollection(ATCC)a