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抗PEG10单克隆抗体的制备及在肝癌中的初步应用.docx

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抗PEG10单克隆抗体的制备及在肝癌中的初步应用 Abstract PEG10isarecentlydiscoveredoncofetalgenethatisoverexpressedinavarietyofsolidtumors,includinglivercancer.Inthisstudy,wepreparedamonoclonalantibodyagainstPEG10andexploreditspotentialapplicationinlivercancer. Methods ThePEG10monoclonalantibodywasgeneratedbyimmunizingmicewithaPEG10recombinantprotein.Afterscreeningandcloningofhybridomacells,themonoclonalantibodywaspurifiedandcharacterizedbywesternblot,ELISA,andimmunofluorescenceassays.TheexpressionofPEG10inlivercancercelllinesandtissueswasalsoanalyzedbywesternblotandimmunohistochemicalstaining. Results AmonoclonalantibodyagainstPEG10wassuccessfullypreparedandpurified,whichspecificallyrecognizedPEG10proteininwesternblotandELISAassays.ImmunofluorescencestainingrevealedthatPEG10localizedinthecytoplasmoflivercancercells.PEG10expressionwassignificantlyhigherinlivercancercelllinesandtissuesthaninnormallivercellsandtissues,anditsexpressionwaspositivelycorrelatedwiththepathologicalgradeoflivercancer. Conclusion WehavesuccessfullygeneratedamonoclonalantibodyagainstPEG10anddemonstrateditsspecificityandsensitivityfordetectingPEG10proteininlivercancercellsandtissues.OurpreliminaryresultssuggestthatPEG10mayserveasapotentialdiagnosticandtherapeutictargetforlivercancer.FurtherstudiesareneededtoexploretheclinicalapplicationsofPEG10monoclonalantibodyinlivercancer. Introduction Livercancerisoneofthemostcommonmalignanttumorsworldwidewithahighincidencerateandmortalityrate.Despiteadvancesindiagnosisandtreatment,livercancerstillposesasignificantthreattohumanhealth.Currently,thediagnosisandtreatmentoflivercancermainlyrelyonimagingtechniquesandserumbiomarkers,suchasalpha-fetoprotein(AFP).However,thesemethodshavelimitationsinsensitivityandspecificity,andnoveldiagnosticandtherapeutictargetsareneededtoimprovetheoutcomesoflivercancerpatients. PEG10isarecentlyidentifiedoncofetalgenethatisnormallyexpressedintheplacentabutisalsooverexpressedinvarioussolidtumors,includinglivercancer.PEG10hasbeenshowntopromotecellprolife