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拟南芥Formin同源蛋白AtFH16的亚细胞定位及功能初探.docx

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拟南芥Formin同源蛋白AtFH16的亚细胞定位及功能初探 摘要 拟南芥（Arabidopsisthaliana）是一种被广泛研究的模式植物。在拟南芥中，存在着许多关键基因，其中包括调控细胞骨架的基因。Formin同源蛋白AtFH16是这些基因中的一个，该蛋白具有调节微丝形态和发育的功能。在本研究中，利用基因编辑技术得到了基因突变体，并通过荧光探针观察AtFH16在拟南芥中的亚细胞定位。结果表明，AtFH16主要在细胞质中分布，并参与了细胞内微丝的组装。此外，通过对AtFH16基因在植物生长发育中的功能进行实验验证，结果表明AtFH16参与了拟南芥花器官的发育。该研究为进一步理解拟南芥生长发育中细胞骨架的调控机制提供了新的思路。 Abstract Arabidopsisthalianaisawidelystudiedmodelplant.AmongthekeygenesinArabidopsis,therearegenesthatregulatethecytoskeleton.ForminhomologyproteinAtFH16isoneofthesegenes,whichisresponsibleforregulatingmicrofilamentmorphologyanddevelopment.Inthisstudy,agenemutantwasgeneratedusinggeneeditingtechnology,andthesubcellularlocalizationofAtFH16inArabidopsiswasobservedusingafluorescenceprobe.TheresultsshowedthatAtFH16wasmainlydistributedinthecytoplasmandparticipatedintheassemblyofmicrofilamentsinthecell.Inaddition,experimentalverificationoftheAtFH16geneinplantgrowthanddevelopmentshowedthatAtFH16isinvolvedinthedevelopmentofArabidopsisflowerorgans.ThisstudyprovidesnewideasforfurtherunderstandingtheregulationmechanismofcytoskeletoninArabidopsisgrowthanddevelopment. Introduction Thecytoskeletonplaysanimportantroleinthegrowthanddevelopmentoforganisms.Inplants,thecytoskeletoniscomposedofmicrofilaments,microtubules,andintermediatefilaments.Microfilamentsarethethinnestcomponentofthecytoskeleton,withadiameterofabout7nm.Themaincomponentofmicrofilamentsisactin,whichisalsothemostabundantproteinincells.Forminsareaclassofproteinsthatregulatethepolymerizationofactinfilaments.AtFH16isaForminhomologyproteinthatregulatesthemorphologyofmicrofilamentsandisinvolvedinplantdevelopment. Inthisstudy,geneeditingtechnologywasusedtoobtainAtFH16genemutantplants,andthesubcellularlocalizationofAtFH16wasobservedusingafluorescenceprobe.ThefunctionofAtFH16inplantgrowthanddevelopmentwasexploredthroughexperiments. Materialsandmethods GenerationofAtFH16genemutantplants TheCRISPR/Cas9geneeditingtechnologywasusedtoobtainAtFH16genemutantplants.ThesgRNAsequencetargetingtheAtFH16genewasdesignedandsynt