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大豆芽菜中游离氨基酸和维生素C含量的遗传分析及QTL定位研究.docx

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大豆芽菜中游离氨基酸和维生素C含量的遗传分析及QTL定位研究 摘要 大豆芽菜richinproteinandvitaminC，isapopularvegetableinChina.Inthisstudy,weconductedageneticanalysisofthecontentsoffreeaminoacidsandvitaminCinsoybeansproutsusingageneticmappingstrategy.WeidentifiedrelatedQTLsandcandidategenesresponsibleforthesynthesisandaccumulationoffreeaminoacidsandvitaminCinsoybeansprouts.Thisstudyprovidedatheoreticalbasisforthegeneticimprovementofsoybeansproutsinthefuture. 关键词：大豆芽菜；游离氨基酸；维生素C；遗传分析；QTL定位 Introduction Soybeansproutsarerichinproteinandvitamins,especiallyvitaminC,andareverypopularinChina.Inadditiontotheirnutritionalvalue,soybeansproutsalsohavegoodmedicinalpropertiesandcanbeusedtotreatdiseases,suchasdiabetes,hypertension,andcardiovasculardiseases(Yeetal.,2013).However,thequalityofsoybeansproutsisaffectedbymanyfactors,suchasthevarietyofsoybean,thegrowthconditions,andtheharvesttime.Therefore,itisnecessarytoimprovethequalityofsoybeansproutsthroughgeneticimprovement. FreeaminoacidsandvitaminCareimportantnutritionalcomponentsofsoybeansprouts,andtheircontentsarecloselyrelatedtothequalityofsoybeansprouts.PreviousstudieshaveshownthatthecontentsoffreeaminoacidsandvitaminCinsoybeansproutsareaffectedbygenetic,environmental,andotherfactors(Lietal.,2015;Yanetal.,2020).However,thegeneticbasisofthefreeaminoacidandvitaminCcontentsinsoybeansproutsisstillunclear. Inthisstudy,weconductedageneticanalysisofthecontentsoffreeaminoacidsandvitaminCinsoybeansproutsusingageneticmappingstrategy.WeidentifiedrelatedQTLsandcandidategenesresponsibleforthesynthesisandaccumulationoffreeaminoacidsandvitaminCinsoybeansprouts.Thisstudyprovidedatheoreticalbasisforthegeneticimprovementofsoybeansproutsinthefuture. MaterialsandMethods Plantmaterials Inthisstudy,weusedtheF2populationderivedfromthecrossbetweenasoybeancultivarwithhighfreeaminoacidandvitaminCcontentsandanothercultivarwithlowfreeaminoacidandvitaminCcontents.TheF2populationconsistedof300individuals,andtheirseedswerecollectedandstoredforfurtheranalysis. Measurementoffreeaminoacidandvit