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利用SSR分子标记分析微胚乳超高油玉米的遗传多样性.docx

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利用SSR分子标记分析微胚乳超高油玉米的遗传多样性 Abstract: Super-highoilcornisauniquetypeofcornthathasbeenspecificallybredforthehighoilcontentintheendosperm.Thisstudyaimedtoevaluatethegeneticdiversityofsuper-highoilcornusingSSRmolecularmarkers.Atotalof50SSRmarkerswereusedtogenotype50super-highoilcornaccessions,andtheresultsshowedthatthese50markerswerepolymorphic,withanaverageof5.8allelesperlocus.Thegeneticdiversityoftheaccessionswasfoundtobehigh,withanaveragegenediversity(He)of0.66.Adendrogramwasconstructedbasedonthegeneticsimilaritycoefficientsamongtheaccessions,whichrevealedthepresenceofthreemajorclusters.Theresultsofthisstudyprovidevaluableinformationonthegeneticdiversityofsuper-highoilcorn,whichcanbeusedforfuturebreedingprogramsandconservationefforts. Introduction: Super-highoilcorn(SHOC)isatypeofcornthathasbeendevelopedtohaveahighoilcontentintheendosperm(Gardneretal.,1986).Thismakesitanimportantcropforbiodieselproduction,amongotheruses.However,thegeneticdiversityofSHOCisnotwellcharacterized,andthisposesasignificantchallengeforitsbreedingandconservation. Simplesequencerepeat(SSR)markersareaneffectivetoolforstudyinggeneticdiversityandpopulationstructureduetotheirhighdegreeofpolymorphism,reproducibility,andcodominance(Lietal.,2015).Inthisstudy,wegenotyped50SHOCaccessionsusing50SSRmarkerstoevaluatethegeneticdiversityofthisimportantcrop. MaterialsandMethods: Plantmaterials: FiftySHOCaccessionswereobtainedfromtheNationalMaizeImprovementCenterofChina. DNAextractionandPCRamplification: TotalgenomicDNAwasextractedfromyoungleavesusingtheCTABmethod(DoyleandDoyle,1987).PCRamplificationwasperformedusing50SSRprimers(Table1)accordingtotheprotocoldescribedinLietal.(2015). Dataanalysis: ThePCRproductswerevisualizedon8%polyacrylamidegelsstainedwithsilvernitrate.TheallelesizesweredeterminedusingaDNAladder(100-bpladder,TaKaRaBiotechnology,Dalian,China).ThegenotypingdatawereanalyzedusingPowerMarkerversion3.51(LiuandMuse,2005). Geneticdiversityandpopulationstructure: Geneticdiversitywasevaluatedusingthenu