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冻融肿瘤疫苗联合顺铂对小鼠恶性黑色素瘤抗瘤作用的实验研究.docx

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冻融肿瘤疫苗联合顺铂对小鼠恶性黑色素瘤抗瘤作用的实验研究 Abstract Objective:Theobjectiveofthisstudyistoinvestigatetheantitumoreffectofcombinedcryoimmunotherapyandcisplatininmicewithmalignantmelanoma. Methods:Atotaloffortymicewererandomlydividedintofourgroups:controlgroup,cryoimmunotherapygroup,cisplatingroupandcombinationtherapygroup.Tumorgrowthrate,survivaltime,histopathologicalchangesandimmunefunctionwereevaluated. Results:Thecombinationtherapygroupshowedthelowesttumorgrowthrateandhighestsurvivalrate.Tumornecrosisandapoptosisweresignificantlyincreasedinthecombinationtherapygroupcomparedtotheothergroups.Combinationtherapyalsosignificantlyenhancedtheimmunefunctionofthemice. Conclusions:Cryoimmunotherapycombinedwithcisplatinhasasignificantantitumoreffectonmicewithmalignantmelanoma,andmaybeapromisingtreatmentstrategyforthisdisease. Keywords:Cryoimmunotherapy,cisplatin,malignantmelanoma,antitumoreffect. Introduction Melanomaisatypeofmalignantskincancerthatarisesfromthemelanocytes,whichareresponsibleforproducingthepigmentthatgivesskinitscolor.Itisahighlyaggressivetumorwithahighpotentialformetastasisandapoorprognosis.Despiteadvancesintreatmentoptions,themortalityrateofmelanomaremainshigh,makingthedevelopmentofeffectivetherapiesatoppriority. Cryoimmunotherapyisanoveltreatmentstrategythatcombinescryosurgerywithimmunotherapy.Itinvolvestheuseofextremecoldtofreezeanddestroythetumor,whilesimultaneouslystimulatingtheimmunesystemtoattackcancercells.CisplatinisacommonlyusedchemotherapeuticagentthatactsbydisruptingthereplicationandrepairofDNAincancercells.Ithasbeenshowntohaveasignificantantitumoreffectinmelanoma,eitherasasingleagentorincombinationwithotherdrugs. Inthisstudy,weaimedtoinvestigatetheantitumoreffectofcombinedcryoimmunotherapyandcisplatininamousemodelofmalignantmelanoma,andtoexploreitsunderlyingmechanisms. MaterialsandMethods Animalmodel FortyBALB/cmice(female,aged6-8weeks)werepurchasedfromBeijingVitalRiverLaboratoryAnimalTechnologyCo.,Ltd.ThestudywasconductedinaccordancewiththeguidelinesoftheIns