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利用代谢组学研究生殖系统调控秀丽线虫衰老的机制.docx

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利用代谢组学研究生殖系统调控秀丽线虫衰老的机制 Title:InvestigatingtheMechanismsofReproductiveSystemRegulationonAginginCaenorhabditiselegansthroughMetabolomics Abstract: Agingisacomplexprocessinfluencedbyvariousfactors,includinggeneticandenvironmentalfactors.Thereproductivesystemhasbeensuggestedtoplayacrucialroleinmodulatingtheagingprocess.Caenorhabditiselegans,anestablishedmodelorganism,allowsustostudythemechanismsbywhichthereproductivesystemregulatesaging.Inthisstudy,weemployedmetabolomics,apowerfultoolforstudyingthecompletesetofmetabolitesinanorganism,toinvestigatethemetabolicchangesassociatedwithreproductivesystemregulationofaginginC.elegans.Ourfindingsshedlightontheunderlyingmechanismsandpotentialtherapeutictargetsforage-relateddiseases. Introduction: Agingisamultifactorialbiologicalprocesscharacterizedbyagradualdeclineinphysiologicalfunctionsandincreasedsusceptibilitytodiseases.Understandingthemechanismsunderlyingagingiscrucialfordevelopinginterventionstodelayage-relateddeclineandincreasehealthspan.Thereproductivesystemhasbeenimplicatedasamodulatoroftheagingprocess,withvariousstudieshighlightingitsroleininfluencinglifespan.However,theexactmechanismsbywhichthereproductivesystemregulatesagingremainelusive.Inthisstudy,weaimedtoinvestigatethemetabolicchangesassociatedwithreproductivesystemregulationofaginginthemodelorganismC.elegansusingmetabolomics. Methods: 1.ExperimentalSetup: WemaintainedC.elegansunderstandardlaboratoryconditionsanddividedthemintothreegroups:wild-typehermaphrodites,sterilehermaphrodites,andmales.Thelifespanofeachgroupwasmonitored,andsampleswerecollectedatmultipletimepointsformetabolomicsanalysis. 2.MetabolomicsAnalysis: MetaboliteswereextractedfromC.eleganssamplesusingastandardizedprotocol.Liquidchromatography-massspectrometry(LC-MS)wasemployedtoanalyzetheextractedmetabolites.Datawereprocessedusingappropriatesoftware,andmetaboliteidentificationandquantificationwereperformedbasedonestablishedmetabolitedatabases.Statisticalanalyseswereconductedtoidentifydifferentiallyr