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微生物沉积碳酸钙封堵混凝土裂缝的试验研究 Abstract: Theexperimentonmicrobialinducedcalciumcarbonateprecipitationforsealingconcretecrackswascarriedout.Thestudyaimedtoexplorethefeasibilityandeffectivenessofusingmicroorganismstoaddressconcretecrackingproblems.Theexperimentaldesigninvolvedtheuseoffivegroupsofbacterialagents,differentconcentrationratiosofcalciumlactateandurea,andvariouscrackwidths.Resultsshowedthattheapplicationofmicrobialinducedcalciumcarbonateprecipitation(MICP)effectivelysealedthecracks,andthemosteffectivebacterialagentswereBacillussubtilisandSporosarcinapasteurii.Moreover,thehighertheconcentrationofcalciumlactateandurea,thebetterthesealingeffect.Thisresearchprovidesasustainable,cost-effectiveandenvironmentallyfriendlysolutionintheconstructionindustry. Keywords:microbialinducedcalciumcarbonateprecipitation,concretecracking,bacterialagents,calciumlactate,urea Introduction: Concretecrackingisacommonproblemincivilengineeringandconstruction.Cracksinconcretestructurescanaffectitsstability,andifleftunattended,itcanleadtoseriousproblemslikewaterleakage,corrosionofthereinforcementsteelandstructuralfailure.Traditionally,sealingagentssuchasepoxiesandpolyurethanesareusedtorepaircracks.However,theseagentshavelimitationssuchashighcost,toxicity,andlowdurability. Inrecentyears,therehasbeengrowinginterestintheuseofmicroorganismsforrepairingconcretecracks.Microbialinducedcalciumcarbonateprecipitation(MICP)isabiomineralizationprocesswheremicroorganismsareusedtodepositcalciumcarbonateinthecracks.Theprocessinvolvesusingbacterialagentstoenhancetheprecipitationofcalciumcarbonate,whichfillsupthecracks. TheuseofMICPhasmanyadvantagesovertraditionalsealingagents.Itiscost-effective,environmentallyfriendly,andprovidesasustainablesolution.Furthermore,itincreasesthedurabilityandstrengthoftheconcretestructure.However,theapplicationofMICPforsealingconcretecracksisstillintheresearchstage,andfurtherexperimentationisnecessarytofullyunderstanditsfeasibilityandeffectiveness. ExperimentalDesign: Inthisexperim