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MSC调控DCs对ARDS小鼠肺部炎症反应影响的研究.docx

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MSC调控DCs对ARDS小鼠肺部炎症反应影响的研究 Title:TheInfluenceofMSCRegulationontheInflammatoryResponseintheLungsofARDSMice:AStudy Abstract: Acuterespiratorydistresssyndrome(ARDS)isasevereinflammatorylungdisorderwithhighmorbidityandmortality.Thepresentstudyaimedtoinvestigatetheimpactofmesenchymalstemcell(MSC)regulationontheinflammatoryresponseinthelungsofARDSmice.MurinemodelsofARDSwereestablishedbyintratracheallipopolysaccharide(LPS)instillation,andMSCswereadministeredviatailveininjection.Theprogressionoflunginflammationwasevaluatedbyanalyzingbiochemicalmarkers,histopathologicalchanges,cytokinelevels,andcellinfiltrationinlungtissues.ThetherapeuticeffectsofMSCsoninflammationwereassessed,providingvaluableinsightsintothepotentialofMSC-basedtherapyforARDStreatment. Introduction: Acuterespiratorydistresssyndrome(ARDS)isasevererespiratorydisordercharacterizedbydiffusealveolardamage,leadingtoimpairedgasexchangeandsubsequentrespiratoryfailure.ThepathogenesisofARDSinvolvesadysregulatedinflammatoryresponseinthelung,resultingindamagetothealveolar-capillarybarrierandexcessiveinfiltrationofinflammatorycells.Despiteextensiveresearch,themanagementofARDSremainsachallenge. Mesenchymalstemcells(MSCs)haveemergedaspromisingcandidatesforthetreatmentofARDSduetotheirimmunomodulatoryandregenerativeproperties.MSCspossesstheabilitytomodulatetheinflammatoryresponseandsupporttissuerepair,makingthemattractivefortherapeuticinterventioninARDS.However,theprecisemechanismbywhichMSCsregulatelunginflammationinthecontextofARDSrequiresfurtherinvestigation. Methods: MaleC57BL/6miceweredividedrandomlyintothreegroups:control,ARDS,andMSC-treatedARDS.ARDSwasinducedinmicebyintratrachealinstillationoflipopolysaccharide(LPS).MSCswereobtainedfromhealthymiceandadministeredviatailveininjection.ControlmicereceivedsalineinsteadofLPS.Lungswereharvestedatspecifictimepointsforanalysis. Results: TheintratrachealinstillationofLPSinducedarobustinflammatoryresponseinthelungs,asevidencedbyincreasedlevelsofpro-inflammatorycytokines(suchastum