预览加载中，请您耐心等待几秒...

BTG2基因抑制卵巢肿瘤细胞增殖和转移及其相关机制研究.docx

预览
1/3
2/3
3/3

在线预览结束，喜欢就下载吧，查找使用更方便

下载提示 文本预览

如果您无法下载资料，请参考说明：

1、部分资料下载需要金币，请确保您的账户上有足够的金币

2、已购买过的文档，再次下载不重复扣费

3、资料包下载后请先用软件解压，在使用对应软件打开

BTG2基因抑制卵巢肿瘤细胞增殖和转移及其相关机制研究 Title:InhibitionofBTG2GeneSuppressesOvarianTumorCellProliferation,Migration,andtheUnderlyingMechanisms Abstract: Ovariancanceristhemostlethalgynecologicalmalignancyworldwideduetoitsaggressivegrowth,highmetastaticpotential,andlimitedtreatmentoptions.Therefore,itisessentialtoidentifynoveltherapeutictargetsfortheeffectivemanagementofovariancancer. ThisstudyaimstoinvestigatetheroleofBTG2(B-celltranslocationgene2)intheregulationofovariantumorcellproliferationandmetastasis,aswellastheunderlyingmechanismsinvolved. Keywords:BTG2,ovariancancer,proliferation,migration,metastasis,therapeutictarget. 1.Introduction: Ovariancancerisahighlymalignanttumorwithahighmortalityrateduetoitslatediagnosisandlimitedtreatmentoptions.Therefore,identifyingnewtherapeutictargetsiscrucialtoimproveoutcomesforovariancancerpatients.BTG2,alsoknownasNGFIA-1BorPC3,isamemberoftheBTG(B-celltranslocationgene)family,whichhasbeenimplicatedinvariouscellularprocesses,includingproliferation,differentiation,andapoptosis.WhileBTG2hasshowntumor-suppressiveeffectsinseveralcancertypes,itsroleinovariancancerremainslargelyunexplored. 2.BTG2andOvarianTumorCellProliferation: 2.1TheexpressionofBTG2inovariantumorcells: ToinvestigatethepotentialroleofBTG2inovariancancer,weanalyzeditsexpressionlevelsinovariantumorcelllinesandclinicaltumorsamplesusingimmunohistochemistryandwesternblotanalysis.OurresultsdemonstratedasignificantlydecreasedexpressionofBTG2inovariantumorcelllinescomparedtonormalovarianepithelialcells. 2.2BTG2-mediatedregulationofovariantumorcellproliferation: ToevaluatetheimpactofBTG2onovariantumorcellproliferation,weperformedgain-andloss-of-functionassays.OverexpressionofBTG2inovariantumorcelllinesledtoasignificantreductionincellviability,cellcyclearrestattheG0/G1phase,andincreasedapoptosis.Conversely,theknockdownofBTG2expressionpromotedcellproliferation. 3.BTG2andOvarianTumorCellMigrationandMetastasis: 3.1TheassociationbetweenBTG2expressionandovariantumormetastasis: Todeterminethec