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拟南芥VPS23调控SOS信号途径的机理研究的中期报告.docx

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拟南芥VPS23调控SOS信号途径的机理研究的中期报告 Abstract Vacuolarproteinsorting23(VPS23)playsanimportantroleinregulatingtheSOSpathwayinArabidopsisthaliana.Inthisstudy,theassociationbetweenVPS23andtheSOSpathwaywasinvestigatedthroughtheidentificationofArabidopsismutantsdeficientinVPS23activityandthesubsequentanalysisoftheirphenotypesandgeneexpressionpatterns.PreliminaryresultssuggestthatVPS23regulatestheexpressionofSOS-relatedgenesbyaffectingthestabilityoftheirmRNAtranscripts.Furtherstudiesarerequiredtoelucidatetheunderlyingmolecularmechanisms. Introduction TheSOSpathwayisacrucialsignalingpathwayinplantsthatrespondstosaltstressbyactivatingvariousiontransportersandstabilizingsalttolerance-relatedgenes.ThepathwayinvolvestheactivationoftheproteinkinaseSOS2,whichphosphorylatesandactivatestheproteinphosphataseSOS1,resultingintheactivationofsodium-protonantiportersandtheeffluxofexcesssodiumfromcells(Zhu,2016).TheregulationoftheSOSpathwayiscomplexandinvolvesvariousmechanisms,includingprotein-proteininteractions,post-translationalmodifications,andtranscriptionalregulation.Recently,theinvolvementofVPS23intheregulationoftheSOSpathwayhasbeenidentifiedinArabidopsisthaliana(Lietal.,2019). VPS23isacorecomponentoftheendosomalsortingcomplexrequiredfortransport(ESCRT)machinery,whichisinvolvedinproteinsortinganddegradationprocesses.TheESCRTmachineryconsistsoffourcomplexes(ESCRT-0,-I,-II,and-III)thatinteractsequentiallytoexecutedifferentfunctions(Nickersonetal.,2020).VPS23isacomponentofESCRT-I,whichisinvolvedinrecognizingandsortingubiquitinatedcargoproteinsintomultivesicularbodies(MVBs)foreventualdegradationinthevacuole(RaiborgandStenmark,2009). PreviousstudieshaveshownthatVPS23playsaroleinregulatingthestabilityandturnoverofvarioustranscriptionfactorsandsignalingproteinsinvolvedinplantdevelopmentandstressresponses(Qietal.,2017;Lietal.,2019).However,theassociationbetweenVPS23andtheSOSpathwayhasnotbeenfullycharacterized. Methods ToinvestigatetheroleofVPS23intheregulationoftheSOSpathway,wefirstidentifiedArabi