黑龙江省瓜类白粉病菌生理小种鉴定及其多样性分析.docx

黑龙江省瓜类白粉病菌生理小种鉴定及其多样性分析
Abstract
Powderymildewdiseaseincucurbitsiscausedbyspecificfungi,whichcancauseseveredamagetocucurbitcrops.Inthisstudy,weaimedtoidentifyandanalyzethephysiologicalracesofpowderymildewfungiincucurbitsanddeterminetheirdiversityinHeilongjiangProvince.WeanalyzedsamplesfromdifferentregionsofHeilongjiangProvinceandidentifiedthephysiologicalracesofpowderymildewfungiusingKoch'spostulates.DNAsequencingwasperformedtodeterminethemoleculardiversityofthefungi.
Theresultsrevealedthatthecucurbitpowderymildewfungicouldbeclassifiedintothreephysiologicalraces.Race1wasthemostfrequent,andwaspresentinallregionsofHeilongjiangProvince.Races2and3werelesscommon,andwereonlypresentincertainareas.MoleculardiversityanalysisbasedonDNAsequencingrevealedthepresenceoftwogroupsoffungi,onedominatedbyPodosphaeraxanthii,andtheotherbyGolovinomycescichoracearum.
ThisstudyprovidesimportantinformationonthediversityanddistributionofcucurbitpowderymildewfungiinHeilongjiangProvince,whichcanbeusedforthedevelopmentofeffectivemanagementstrategiesforthedisease.
Introduction
Cucurbitsareanimportantgroupofcropswitheconomicandnutritionalvalue,whicharewidelygrowninmanyregionsoftheworld.However,cucurbitcropsareoftenaffectedbyvariouspestsanddiseases,whichcanresultinsignificantyieldlosses.Powderymildewisacommondiseaseincucurbits,whichiscausedbyspecificfungithatcaninfectvariouspartsoftheplant,includingleaves,stems,flowers,andfruits.Thesymptomsofpowderymildewincludetheformationofwhitepowderypatchesontheinfectedplantparts,whichcanspreadrapidlyandcauseseveredamagetothecrop.
CucurbitpowderymildewfungibelongtotheorderErysiphales,whichincludesmanydifferentgeneraandspecies.ThemostcommongeneraofpowderymildewfungiincucurbitsarePodosphaeraandGolovinomyces.Powderymildewfungihavebeentraditionallyclassifiedbasedontheirhostrange,morphology,andphysiologicalcharacteristics.However,thesemethodshavelimitationsindiscriminatingcloselyrelatedspecies,andoftendonotreflectthetruegeneticdiversityoffungi.
Molecularmethods,suc