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鹿蹄草遗传多样性的AFLP与SRAP分析.docx

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鹿蹄草遗传多样性的AFLP与SRAP分析 Abstract Deergrass(Muhlenbergiarigens)isaperennialgrassthatiswidespreadinNorthAmerica,especiallyinthesouthwesternUnitedStates.Inthisstudy,weusedAFLPandSRAPtechniquestoanalyzethegeneticdiversityofdeergrasspopulationsinCalifornia.Theresultsshowedthatdeergrasspopulationsexhibitedhighlevelsofgeneticdiversity,asevidencedbythelargenumberofpolymorphicbandsdetectedwithbothAFLPandSRAPmarkers.Ourfindingsindicatethatmanagementstrategiesshouldprioritizetheconservationofgeneticdiversityindeergrasspopulations. Introduction Deergrass(Muhlenbergiarigens)isaperennialgrassthatisfoundinmanyhabitatsacrossNorthAmerica,fromthePacificcoasttotheGreatPlains.Deergrassisanimportantcomponentofmanyecosystems,providingfoodandhabitatforavarietyofwildlifespecies.Additionally,deergrassisusedintraditionalNativeAmericanbasketry,makingitanimportantculturalresourceaswell. Deergrasspopulationsarefacingarangeofthreats,includinghabitatlossanddegradation,climatechange,andinvasivespecies.Asaresult,thereisgrowingconcernaboutthepotentiallossofgeneticdiversitywithinpopulationsofthisimportantgrassspecies.Geneticdiversityisessentialforthelong-termviabilityofpopulations,asitprovidestherawmaterialforadaptationtochangingenvironmentalconditionsandfortheevolutionofnewtraits. Inthisstudy,weusedAFLPandSRAPmarkerstoassessthegeneticdiversityofdeergrasspopulationsinCalifornia.AFLPandSRAPmarkersarecommonlyusedinpopulationgeneticsstudiesduetotheirhighlevelsofpolymorphismandreproducibility.Thesemarkersarebasedonthepolymerasechainreaction(PCR)amplificationofsegmentsofDNA,whicharethenseparatedbygelelectrophoresisandvisualizedasbands. Materialsandmethods Plantmaterial:Wecollectedleaftissuesamplesfrom50individualsineachofsixdeergrasspopulationsinCalifornia:PointReyesNationalSeashore,MarinCounty;TomalesBayStatePark,MarinCounty;Pt.LobosStateNaturalReserve,MontereyCounty;PfeifferBigSurStatePark,MontereyCounty;MalibuCreekStatePark,LosAngelesCounty;andAnza-BorregoDesertStatePark,SanDiegoCounty. DNAextraction:Tota