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经脾脏移植BMP-4诱导分化的肝干细胞治疗大鼠急性肝衰竭的实验研究.docx

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经脾脏移植BMP-4诱导分化的肝干细胞治疗大鼠急性肝衰竭的实验研究 摘要 急性肝衰竭是一种极其危险的疾病，但目前并没有有效的治疗手段。本研究试图通过经脾脏移植BMP-4诱导分化的肝干细胞治疗大鼠急性肝衰竭。结果表明，该治疗方法可以显著地促进肝细胞的再生和修复，改善临床症状，并提高大鼠的存活率。这为治疗急性肝衰竭提供了新的思路和方法。 关键词：肝干细胞、BMP-4、急性肝衰竭、治疗、移植 Abstract Acuteliverfailureisanextremelydangerousdisease,butthereiscurrentlynoeffectivetreatment.ThisstudyattemptedtotreatacuteliverfailureinratsbytransplantingBMP-4-induceddifferentiationofliverstemcellsthroughthespleen.Theresultsshowedthatthistreatmentmethodcansignificantlypromotetheregenerationandrepairoflivercells,improveclinicalsymptoms,andincreasethesurvivalrateofrats.Thisprovidesanewideaandmethodforthetreatmentofacuteliverfailure. Keywords:liverstemcell,BMP-4,acuteliverfailure,treatment,transplantation Introduction Acuteliverfailure(ALF)isaclinicalsyndromecharacterizedbyrapiddevelopmentofliverdysfunctioninpatientswithoutpreviousliverdisease.ThecausesofALFarediverse,includingviralhepatitis,drug-inducedliverinjury,autoimmunehepatitis,andmetabolicliverdiseases.ALFisalife-threateningdiseasethatcanleadtomultiorganfailureanddeath(Schiodtetal.,2001). Currently,thereisnoeffectivetreatmentforALF.LivertransplantationistheonlydefinitivetreatmentforALFpatients,butitislimitedbytheavailabilityofdonororgansandhighcosts.Therefore,thereisanurgentneedtoexplorenewtherapiesforALF. Liverstemcells(LSCs)havetheabilitytodifferentiateintohepatocytesandbileductcells,andcanself-renewinvitroandinvivo(Dongetal.,2020).LSCstransplantationhasbeenshowntobeapromisingtreatmentforliverdiseases,suchasliverfibrosisandcirrhosis(Dingetal.,2020).BMP-4isamemberofthebonemorphogeneticprotein(BMP)family,whichplaysanimportantroleinliverdevelopmentandregeneration(Jungetal.,2011).BMP-4caninducedifferentiationofLSCsintohepatocytes,andpromotetheproliferationandsurvivalofhepatocytes(Zhangetal.,2018).Therefore,transplantationofBMP-4-induceddifferentiationofLSCsmaybeapotentialtreatmentforALF. MaterialsandMethods Animals MaleSprague-Dawleyratsweighing200-250gwereobtainedfromtheAnimalCenterofChinaMedicalUniversity.Allanima