预览加载中，请您耐心等待几秒...

棉铃中主要内源激素含量的表达特性研究.docx

预览
1/3
2/3
3/3

在线预览结束，喜欢就下载吧，查找使用更方便

下载提示 文本预览

如果您无法下载资料，请参考说明：

1、部分资料下载需要金币，请确保您的账户上有足够的金币

2、已购买过的文档，再次下载不重复扣费

3、资料包下载后请先用软件解压，在使用对应软件打开

棉铃中主要内源激素含量的表达特性研究 Introduction Cottonisanimportantcropworldwide,providingtextilefibers,oil,andprotein.Cottonisalsoanimportantmodelplantforstudyingthemechanismoffibercelldevelopment.Thedevelopmentofcottonfiberisregulatedbyacomplexnetworkofhormones,includingauxin,cytokinin,gibberellins,abscisicacid,ethylene,andinositol.Thesehormonesplayimportantrolesintheinitiation,elongation,andmaturationofcottonfiber.Amongthesehormones,auxinisakeyregulatorofcottonfiberdevelopment,whichhasbeenextensivelystudied.However,thefunctionsofotherhormonesinregulatingcottonfiberdevelopmentarepoorlyunderstood.Here,wefocusontheexpressioncharacteristicsofinternalhormonesincottonbolls,especiallyinfibercells. Auxin Auxinisakeyregulatorofcottonfiberdevelopment.TheendogenousIAA(indole-3-aceticacid)contentincottonbollsincreasessignificantlyduringtheovuledevelopmentstageafteranthesis,andpeaksat20dayspost-anthesis(DPA).Theexpressionoftheauxinbiosynthesisgene,GhYUC1,ishighintheinitiationandelongationstagesofcottonfiberdevelopment,whiletheexpressionoftheauxineffluxcarriergene,GhPIN1,ishighintheelongationandmaturationstages.ThecottonGhTIR1(transportinhibitorresponse1)geneencodesaputativeauxinreceptor-likeprotein,whichhasahighexpressionlevelinthedevelopingfiber,suggestingthatauxinissensedandtransduceddirectlyinthefibercells.TransgenicoverexpressionofthecottonGhYUC1genecanacceleratefiberinitiationandelongation,whilesuppressionofGhYUC1expressioninhibitsfiberdevelopment.Theauxinresponsefactor(ARF)familyoftranscriptionfactorsmediatedownstreamresponsestoauxin.ThecottonGhARF2andGhARF8genesarehighlyexpressedintheelongationandmaturationstagesoffiberdevelopment,andoverexpressionofGhARF2andGhARF8canenhancefiberlengthandstrength. Gibberellins Gibberellins(GAs)arealsoimportanthormonesforcottonfiberdevelopment.ThecontentofGA3incottonbollsgraduallyincreasesfromtheinitiationtopeakelongationstage,andthendecreasesduringmaturation.TheexpressionofcottonGAbiosynthesisgenesGhGA20ox2andGhGA3ox2isup-regulatedduringtheearlystageo