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干贝素对戊四氮致痫大鼠海马区GABA-AR表达的影响 Abstract: ThepurposeofthisstudyistoinvestigatetheeffectofscalloppeptideonGABA-ARexpressioninthehippocampusofpentetrazole-inducedepilepticrats.MaleWistarratswererandomlydividedintofourgroups:controlgroup,modelgroup,low-dosescalloppeptidegroup,andhigh-dosescalloppeptidegroup.Theexperimentalresultsshowthatscalloppeptidecansignificantlyreducethefrequencyanddurationofepilepticseizuresinpentetrazole-inducedepilepticrats,andincreasetheexpressionofGABA-ARinthehippocampus.Therefore,itissuggestedthatscalloppeptidemayhaveapotentialtherapeuticeffectonepilepsybyregulatingGABA-ARexpressioninthehippocampus. Keywords:Scalloppeptide,Pentetrazole,Epilepsy,GABA-AR,Hippocampus Introduction: Epilepsyisachronicneurologicaldisordercharacterizedbyrecurrentseizures.Theincidenceofepilepsyishigh,anditseriouslyaffectsthehealthandqualityoflifeofpatients.Atpresent,antiepilepticdrugsarestillthemaintreatmentforepilepsy.However,antiepilepticdrugshavecertainlimitations,suchaspoorefficacy,sideeffects,anddrugresistance,whichaffectthelong-termtreatmentofepilepsy.Therefore,itisofgreatsignificancetoexplorenewdrugsandtreatmentmethodsforepilepsy. Scalloppeptideisapolypeptideextractedfromthemuscleofscallops.Ithasawiderangeofbiologicalactivities,suchasantioxidant,anti-inflammatory,antihypertensive,antithrombotic,andimmunomodulatoryeffects.Inrecentyears,studieshavefoundthatscalloppeptidehasacertaintherapeuticeffectonepilepsythroughvariousmechanisms. Gamma-aminobutyricacid(GABA)isanimportantinhibitoryneurotransmitterinthebrain,whichplaysakeyroleintheregulationofneuronalexcitabilityandtheoccurrenceofseizures.GABAreceptors(GABA-Rs)areGABA-gatedionchannelsthatmediatetheeffectsofGABAonneuronalactivity.Amongthem,GABAtypeAreceptor(GABA-AR)isthemainsubtypeofGABA-Rsinthebrain,anditplaysanimportantroleinthepathogenesisandtreatmentofepilepsy. Pentetrazoleisacommonlyuseddrugtoinduceepilepticseizuresinanimalmodels.Thepentetrazole-inducedepilepsymodelisareliableandreproduciblemodel,whichcansimula