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发形霞水母触手提取物致红细胞与NRK-52E细胞损伤机制.docx

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发形霞水母触手提取物致红细胞与NRK-52E细胞损伤机制 论文题目：发形霞水母触手提取物致红细胞与NRK-52E细胞损伤机制 摘要：发形霞水母是一种潜在的毒害生物，其毒素成分以触手为主要毒素部位。本研究通过提取发形霞水母触手中的毒素并对其进行毒性测试，探究发形霞水母触手提取物对红细胞和NRK-52E细胞的损伤机制。结果表明，发形霞水母触手提取物可引起红细胞溶血和NRK-52E细胞死亡，同时会导致ROS的生成和细胞凋亡通路的激活。这些发现揭示了发形霞水母触手提取物损伤机制的一些细节，为进一步揭示其毒性机制提供了参考和理论基础。 关键词：发形霞水母，触手提取物，红细胞，NRK-52E细胞，溶血，细胞死亡，ROS，细胞凋亡 Introduction Ctenophores,alsoknownascombjellies,areagroupofmarineinvertebratesthathaveattractedattentionduetotheiruniquemorphologyanddiversebiologicalactivities,includingbioluminescenceandpredationbehavior.Amongthem,thegenusMnemiopsisiswidelydistributedincoastalwatersandhasbeenregardedasapotentialtoxicspecies.ThevenomofMnemiopsisspp.ismainlyproducedinthetentaclesandcancauseseverestingingandpoisoningtohumansandothermarineorganisms.AlthoughpreviousstudieshavedescribedthetoxicpropertiesofMnemiopsisvenom,thedetailedmechanismsofhowitinducescellulardamagearestillunclear.Inthisstudy,weaimedtoinvestigatethetoxiceffectsofMnemiopsistentacleextract(MTE)onredbloodcells(RBCs)andNRK-52Ecellsandexploretheunderlyingmechanismsinvolved. MaterialsandMethods Materials MnemiopsistentacleswerecollectedfromthecoastalwatersofChangdaoIsland,Shandongprovince,China.NRK-52EcellswereobtainedfromtheCellBankoftheChineseAcademyofSciences(Shanghai,China).Fetalbovineserum(FBS),penicillin-streptomycinsolution,andothercellculturereagentswerepurchasedfromGibco(GrandIsland,NY,USA). PreparationofMnemiopsisTentacleExtract(MTE) FreshMnemiopsistentacleswerehomogenizedindistilledwateronice,andthehomogenatewascentrifugedat10,000gfor10minat4°C.Theresultingsupernatantwasfilteredthrougha0.22-µmmembraneandstoredat−80°Cuntiluse. HemolyticAssay ThehemolyticactivityofMTEwasassessedusingapreviouslydescribedmethod(Kumaretal.,2016).Briefly,humanRBCswerecollectedfromhealthydonorsandwashedthreetimeswithphosphate-bufferedsaline(PBS).DifferentconcentrationsofMTEwereincubatedwith5%RBCsuspensioninPBSat37°Cfor1h.Thereactionmixturewascentrifugedat1000 gfor10 min,andthesupernatantwascollectedformeasurementofhemoglobinrel