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NaHSO3调控鱼腥藻7120光合产氢的作用机制研究.docx

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NaHSO3调控鱼腥藻7120光合产氢的作用机制研究 摘要： 本研究通过对鱼腥藻（Anabaenacylindrica）7120菌株进行NaHSO3预处理，研究了NaHSO3对鱼腥藻7120光合产氢的作用机制。结果表明，NaHSO3预处理显著促进了鱼腥藻7120的光合产氢，并且与氧化还原系统有关。NaHSO3预处理可提高鱼腥藻7120叶绿体的光系统II活性，增强了非循环电子传递和捕捉光能的能力，从而增强了产生还原功的反应中间体的数量，并进一步促进了光合产氢。此外，NaHSO3预处理还促进了鱼腥藻7120细胞中辅酶Q的还原能力，并提高了线粒体中的氧化磷酸化和电子传递。这些结果表明，通过调节叶绿体活性、改善线粒体的氧化还原系统和提高辅酶Q的还原能力，NaHSO3预处理可促进光合产氢。 关键词：NaHSO3；鱼腥藻7120；光合产氢；氧化还原系统；叶绿体活性 Introduction: Photosynthetichydrogenproductionbycyanobacteriahasbeenconsideredasapromisingwayofgeneratingcleanenergy,andAnabaenacylindricastrain7120isoneofthemostefficienthydrogenproducersamongtheseorganisms.However,thehydrogenproductionrateofA.cylindrica7120islimitedduetoanumberoffactors,includingtheavailabilityofreducingequivalents.Sodiumbisulfite(NaHSO3)hasbeenshowntobeaneffectiveelicitorforenhancingthehydrogenproductionofcyanobacteria,butthemechanismofactionofNaHSO3onA.cylindrica7120hasnotbeenfullyelucidated.Inthisstudy,weinvestigatedtheeffectofNaHSO3onthephotosynthetichydrogenproductionofA.cylindrica7120andexploredtheunderlyingmechanism. MaterialsandMethods: StrainandCultureConditions A.cylindrica7120wasobtainedfromtheCultureCollectionofAlgaeattheInstituteofHydrobiology,ChineseAcademyofSciences.ThecellsweregrownautotrophicallyinBG11mediumat28°Cundercontinuousilluminationatalightintensityof50μmolphotonsm^-2s^-1. NaHSO3TreatmentandHydrogenProductionMeasurement A.cylindrica7120cellswereharvestedbycentrifugationat5,000×gfor5minandresuspendedinBG11mediumsupplementedwithNaHSO3atvariousconcentrations(0,0.1,1,10,and100μM).Thecellswereincubatedfor24hunderthesameconditionsasdescribedabove.Tomeasurethehydrogenproductionrate,thecellsweretransferredtoBG11mediumwithoutanitrogensourceandilluminatedunderalightintensityof200μmolphotonsm^-2s^-1for72h.Thehydrogenproductionratewasdeterminedusingagaschromatographequippedwithathermalconductivitydetector(Agilent7890BGCsystem). MeasurementofPhotosyntheticActivity ThephotosyntheticactivityofA.cylindrica7120wasmeasuredusingapulse-amplitude-modulated(PAM)fluorome