HO-1对香烟烟雾提取物诱导支气管上皮细胞凋亡及CHOP表达的调节作用.docx

HO-1对香烟烟雾提取物诱导支气管上皮细胞凋亡及CHOP表达的调节作用
摘要
本文旨在探讨HO-1对香烟烟雾提取物（cigarettesmokeextract，CSE）诱导支气管上皮细胞（bronchialepithelialcells，BECs）凋亡及C/EBP-homologousprotein（CHOP）表达的调节作用。研究发现，HO-1的过表达显著降低了CSE诱导BECs凋亡和CHOP表达的程度。同时，HO-1的抑制剂锡卡二酸（SnPP）显著增强了CSE诱导BECs凋亡和CHOP表达。这些结果表明，HO-1对CSE诱导的BECs凋亡和CHOP表达具有重要的调节作用。深入研究HO-1与支气管哮喘等疾病的关系，具有重要的理论和实际意义。
关键词：HO-1；香烟烟雾提取物；支气管上皮细胞；凋亡；CHOP表达；调节作用
Abstract
Theaimofthisstudyistoinvestigatetheregulatoryeffectofhemeoxygenase-1(HO-1)oncigarettesmokeextract(CSE)-inducedapoptosisandC/EBP-homologousprotein(CHOP)expressioninbronchialepithelialcells(BECs).TheresultsshowedthatoverexpressionofHO-1significantlyreducedthedegreeofCSE-inducedapoptosisandCHOPexpressioninBECs.Inaddition,theHO-1inhibitor,SnPP,significantlyenhancedCSE-inducedapoptosisandCHOPexpression.TheseresultsindicatethatHO-1playsanimportantregulatoryroleinCSE-inducedapoptosisandCHOPexpressioninBECs.FurtherstudyontherelationshipbetweenHO-1andbronchialasthmahasimportanttheoreticalandpracticalsignificance.
Keywords:HO-1;cigarettesmokeextract;bronchialepithelialcells;apoptosis;CHOPexpression;regulatoryeffect
Introduction
Cigarettesmokingisaleadingcauseofpreventabledeathworldwide.Cigarettesmokecontainsavarietyoftoxicchemicalsthatcandamagetherespiratorysystem,includingbronchialepithelialcells(BECs).BECsarethefirstlineofdefenseintherespiratorytract,andplayacriticalroleinprotectingthelungsfrominhaledtoxicantsandpathogens.However,exposuretocigarettesmokecaninduceBECapoptosis,whichisanimportantfactorinthedevelopmentofchronicobstructivepulmonarydisease(COPD)andbronchialasthma.
Hemeoxygenase-1(HO-1)isastress-responsiveenzymethatcatalysesthedegradationofhemeintocarbonmonoxide(CO),biliverdin,andiron.HO-1hasbeenshowntohaveanti-inflammatory,antioxidant,andanti-apoptoticproperties,andisconsideredtobeacytoprotectiveprotein.However,theregulatoryroleofHO-1incigarettesmoke-inducedBECapoptosisisstillunclear.
C/EBP-homologousprotein(CHOP)isapro-apoptotictranscriptionfactorthatisinducedduringendoplasmicreticulum(ER)stress