大孔吸附树脂纯化刺玫果总黄酮工艺研究.docx

大孔吸附树脂纯化刺玫果总黄酮工艺研究论文II摘要刺玫果学名野蔷薇又名野刺玫果山刺玫果系蔷薇科蔷薇属植物山刺玫的成熟果实。广泛分布于我国的东北地区尤以黑龙江省贮量最为丰富。现代药理学研究表明：刺玫果含有丰富的营养成分及生物活性物质具有健脾理气、养血调经、抗衰老、防治心血管疾病、增强免疫及促智等功效。刺玫果中所含的总黄酮类物质具有预防和治疗高血脂、血栓所致的心脑血管疾病的功效且具有高效、低毒副作用的优势。因此纯化刺玫果中的总黄酮就具有现实的科研意义。目前常见的总黄酮纯化方法有水提醇沉法（水醇法）、醇提水沉法（醇水法）、酸碱法、盐析法、离子交换法和结晶法等。实践表明这些方法也存在一定的局限性。近年出现了一些分离和纯化的新方法如絮凝沉淀法大孔树脂吸附法、超滤法、高速离心法等。本论文采用大孔树脂法纯化刺玫果提取物中的总黄酮。探索洗脱剂、pH值、上样浓度等因素对纯化过程的影响。采用紫外分光光度计法对总黄酮含量进行了分析最终确定了洗脱剂的浓度75%、上样PH为5上样浓度为原液浓度吸附速率为2Bv/h洗脱速率为1Bv/h。该纯化方法简单快捷纯化效率高进一步拓展了刺玫果的应用领域和范围为刺玫果的全面和综合利用提供科学的理论依据。关键词：刺玫果总黄酮大孔树脂法纯化AbstractRosamultifloraisisthescientificnameofRosadavuricapallalsoknownasWildrosafruit.RosamountainfruitthisfruitistheRosamountainfruitofRosaceaegenusRosa.Itisgrowingineast-northChinaespeciallyinprovinceofHeilongjiang.ModernpharmaceuticalresearchshowsthatRosadavuricaPallcontainsrichnutrientsandbioactivesubstancesanti-agingpreventionandtreatmentofcardiovasculardiseaseenhancesimmunityandtheefficacyofnootropicetc.TotalflavonoidsintheRosadavuricaPallpossessthefunctionofpreventionandtreatmentofhighbloodlipidsthrombosisduedtocardiovascularandcerebrovasculardiseases.Ithaspropertieslikehigh-efficiencyandtheadvantagesoflowtoxicitysideeffects.Thusitisworthindoingresearchonrefiningtotalflavonoidsinthefruit.RecentlytherearetheseveralcommonmethodsinpurifyingFlavonoidssuchaswater-extractingalcohol-precipitatingalcohol-extractingwater-precipitatingacid-basemethodsaltdopositingio-exchangeandcrystallizationandsoon.Inpracticethereisthelimitinallthesemethodreported.Somenewmethodsareinvestigatedlikeflocculationprecipitationmacroporousresinabsorptionultrafiltrationhigh-speedcentrifugea-tionandsoon.InthisthesiswereportextractionoftotalflavonoidsfromRosadavuricapallbyusingthemethodofMacroporousresin.ItwasinvestigatedbasingonhowthetypeofEluantPHofsolutionconcentrationof