代谢组学研究技术及应用.pptx

会计学代谢组学主要研究技术Gene一mRNA—Protein—>>Metabolite一、什么是代谢组学Metabolomics存在多个定义，但其精髓是：对一个生物系统的细胞在给定时间和给定条件下所有小分子代谢物质的定量分析(thequantitativemeasurementofalllowmolecularweight
metabolitesinanorganismcellsataspecifiedtimeunderspecificenvironmentalconditions).Metabolomics着重研究的是单个细胞或细胞类型中所有的小分子成分和波动规律,或许可以叫做细胞代谢组学.多用于植物和微生物系统.
Metabonomics代谢组学是关于定量描述生物内源性代谢物质的整体及其对内因和外因变化应答规律的科学.
(Metabonomicsisthebranchofscienceconcernedwiththequantitative
understandingsofthemetabolitecomplementofintegratedlivingsystems
anditsdynamicresponsestothechangesofbothendogenousfactors(suchasphysiologyanddevelopment)andexogenousfactors(suchasenvironmentalfactorsandxenobiotics)).
因此，Metabolomics可以译作代谢物组学.Metabolomics
是Metabonomics的一个组成部分.
3.代谢组学的发展二、代谢组学主要研究技术代谢组学各分析流程技术
/Abstract
Proton-NMR-basedmetabonomicsoffersarareopportunityasadefinitivescreeningtechniqueforbiofluidsandtissuebiopsies.Theprocedureisextraordinaryinthatitallowsthe‘completebiochemicalpicture’tobeexaminedatonetimeandisabletodetectsubtlebutrepeatedlyconsistentdisparitiesthatmaybeoccurringindifferent,andperhapsunrelated,biochemicalpathways.Suchmetabolicresponsestoaninitialperturbationinhomeostasismaybefollowedoverasequentialtime-coursetotheireventualdissipationorconsequentsequelae.Theapplicationofthistechniqueisbeginningslowlytofilterintotheareaofendocrineresearchandhasbeenusedtoexaminelong-termanddiffusephysiologicalalterationsthatmayoccurfollowingsucheventsasanabolicsteroidtreatmentofcattleandtheexposureofendometrialcellstotamoxifen.Althoughonlymodestinroadshavebeenmadesofar,thistechniquepromisesimmensepotentialforfutureresearcheswithintheendocrinefield.Contents
1.Introduction
2.Protonnuclearmagneticresonancespectroscopy
3.Themetabonomicapproachandpatternrecognition
4.Applicationwithinthebiologicalfield
5.Hormones,drugsandtheendocrinesystem
References1.WhatisMetabonomics?
Metabonomics:thequantitativemeasurementofthe
multi-parametricmetabolicresponseoflivingsystemsto
pathophysiologicalstimuliorgeneticmodifications.
(生物系统对生理和病理刺激以及基因改变的代谢应答的