一株海洋异养硝化--好氧反硝化菌的分离鉴定、脱氮特性及应用的中期报告.docx

一株海洋异养硝化--好氧反硝化菌的分离鉴定、脱氮特性及应用的中期报告
摘要：
海洋异养硝化-好氧反硝化菌能够同时实现氨氮和硝酸盐的脱除，是海洋生态系统中重要的脱氮微生物之一。本研究从南海沉积物中分离到一株菌株，经形态特征、16SrDNA序列和生理生化鉴定，确认其为一株海洋异养硝化-好氧反硝化菌。通过对其脱氮特性的研究发现，该菌在碳氮比为11∶1时最适合异养硝化和好氧反硝化，同时在25℃和pH7.5条件下具有最高的脱氮活性。该菌对氨氮和硝酸盐的兼容性较高，有较好的适应性和生长速率。最后，本研究对该菌的应用前景进行了初步探讨，认为其具有广泛的应用前景，尤其在海洋环境中的脱氮方面。
关键词：海洋异养硝化-好氧反硝化菌；分离鉴定；脱氮特性；应用前景
Abstract:
Marineautotrophicnitrifiers-aerobicdenitrifierscanachievesimultaneousremovalofammonianitrogenandnitrate,andareimportantdenitrifyingmicroorganismsinmarineecosystems.Inthisstudy,astrainwasisolatedfromthesedimentoftheSouthChinaSea,andthroughmorphologicalcharacteristics,16SrDNAsequenceandphysiologicalandbiochemicalidentification,itwasconfirmedtobeamarineautotrophicnitrifier-aerobicdenitrifier.Throughthestudyofitsdenitrificationcharacteristics,itwasfoundthatthestrainwasmostsuitableforautotrophicnitrificationandaerobicdenitrificationatacarbon-to-nitrogenratioof11:1,andhadthehighestdenitrificationactivityat25℃andpH7.5.Thestrainhashighcompatibilitywithammonianitrogenandnitrate,andhasgoodadaptabilityandgrowthrate.Finally,thisstudydiscussedtheapplicationprospectsofthestrain,believingthatithasbroadapplicationprospects,especiallyindenitrificationinmarineenvironments.
Keywords:marineautotrophicnitrifiers-aerobicdenitrifiers;isolationandidentification;denitrificationcharacteristics;applicationprospects.