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RecommendedCultureMethodsforMicroorganisms SelectionofGrowthRequirements—————————— 1.Primarygrowthonanonselectiveagarmediumispreferred.Primarygrowthinafluidmedium shouldonlyoccurinspecialinstancesorwhenrecommended.Becauseofthemanipulations requiredduringhydration,itisdifficulttoobtainpurityofalyophilizedstraininafluidmedium.A contaminantmaycompletelyovergrowandobscurethepresenceofthelyophilizedstrain. 2.Thefollowinginformationlistswhichmethodshouldbeusedtogrowthevariousmicroorganism species.Descriptionsofmethodsfollowthemicroorganismlist. MicroorganismMethodNotes AcetobacterspeciesMethod36 AchromobacterspeciesMethod1 AcinetobacterspeciesMethod1 ActinobacillusspeciesMethod3 ActinomycesspeciesMethod4 AerococcusspeciesMethod1 ExceptionsareAeromonashydrophila,Microbiologics0870 AeromonasspeciesMethod2 andAeromonassalmoncida Aeromonashydrophila, Method31 Microbiologics0870 AeromonassalmonicidaMethod32 AggregatibacterspeciesMethod3 AlcaligenesspeciesMethod1 AlicyclobacillusspeciesMethod12AnexceptionisAlicyclobacillusacidoterrestris. AlicyclobacillusacidoterrestrisMethod45 AlloiococcusspeciesMethod2 AlternariaspeciesMethod5 AmylomycesspeciesMethod5 AneurinibacillusspeciesMethod1 AquaspirillumspeciesMethod20. ArcanobacteriumspeciesMethod34 ArthrobacterspeciesMethod21 AspergillusspeciesMethod5AnexceptionisAspergillusflavus. AspergillusflavusMethod46 AureobasidumspeciesMethod5 BacillusspeciesMethod49 BacteroidesspeciesMethod4AnexceptionisBacteroidesureolyticus. BacteroidesureolyticusMethod38 BifidobacteriumspeciesMethod4AnexceptionisBifidobacteriumanimalissubsp.animalis. TIB.081RevisionJPage1of22 MicroorganismMethodNotes Bifidobacteriumanimalis Method39 subsp.animalis. BordetellabronchisepticaMethod15 BordetellaparapertussisMethod16 ExceptionsareBordetellapertussis,Microbiologicscatalog BordetellapertussisMethod16 numbers0100and0843. Bordetellapertussis Method57 Microbiologics0100and0843 BrevibacillusspeciesMethod1 BrevundimonasspeciesMethod1 BrochothrixspeciesMetho