水中微囊藻毒素-LR分子印迹传感技术研究的中期报告.docx

水中微囊藻毒素-LR分子印迹传感技术研究的中期报告
摘要：
微囊藻毒素是一类常见的水生毒素之一，其中微囊藻毒素-LR（MC-LR）由于具有较强的毒性和稳定性，成为了其中研究的热点。本文采用分子印迹技术，以MC-LR为模板分子，通过分别选择甲基丙烯酸甲酯和丙烯酰胺为单体，以过氧化苯甲酰（benzoylperoxide,BPO）为引发剂，制备了分别具有甲基丙烯酸甲酯（MMA）和丙烯酰胺（AM）的聚合物对MC-LR进行分子印迹。采用紫外可见分光光度计测定不同浓度的MC-LR溶液的吸收光谱，通过引入印迹聚合物，在溶液中分别测定印迹聚合物/控制聚合物的吸收光谱，计算了吸光度差值，并绘制了印迹聚合物对MC-LR的选择性系数曲线。结果表明，MMA和AM分别制备的印迹聚合物对MC-LR具有不同的选择性系数，且具有良好的再现性和稳定性。
关键词：分子印迹技术；微囊藻毒素-LR；聚合物；选择性系数
Introduction
Microcystinsbelongtotheclassofcyanotoxins,whicharetoxicsecondarymetabolitesproducedbycyanobacteria.Thesetoxinshavebeenidentifiedinaquaticenvironmentsthroughouttheworld,andareaconcernduetotheirpotentialimpactonhumanandanimalhealth.Microcystin-LR(MC-LR)isoneofthemostcommonlydetectedandtoxicvariantsofthesecyanotoxins.Therefore,itiscrucialtodevelopmethodsfordetectingandquantifyingMC-LRinenvironmentalsamples.
Molecularlyimprintedpolymers(MIPs)aresyntheticmaterialsthatcanselectivelyrecognizetargetmoleculesduetotheir3Dshapeandfunctionalgrouparrangement,similartoanantibody-antigeninteraction.Inrecentyears,MIPshavebeenincreasinglyutilizedforthedetectionofvariousmolecules,includingtoxins.Inthisstudy,weaimedtodevelopamolecularlyimprintedsensorforthedetectionofMC-LRinwatersamplesusingastrategybasedontheprinciplesofmolecularimprinting.
MaterialsandMethods
PreparationofMIPs
MIPsweresynthesizedbybulkpolymerizationusingmethylmethacrylate(MMA)oracrylamide(AM)asthefunctionalmonomers,MC-LRasthetemplatemolecule,andbenzoylperoxide(BPO)astheinitiator.Thepolymerizationsolutionwaspouredintoglasstubesandpolymerizedat60°Cfor24h.TheMIPparticleswerethencrushedandsievedtoobtainparticleswithasizerangeof300-450μm.
CharacterizationofMIPs
ThepropertiesofMIPswerecharacterizedbyscanningelectronmicroscopy(SEM),Fourier-transforminfrared(FTIR)spectra,andsurfaceareameasurements.TheselectivebindingperformanceofMIPswasevaluatedbycomparingtheadsorptioncapacityofMIPstonon-imprintedcontrolpolymers(NIPs).AbsorptionspectraweremeasuredusingaUV-Visspectrophotometer.
ResultsandDiscussion
SEMimagesshowedthatMIPpart