预览加载中,请您耐心等待几秒...
1/2
2/2

在线预览结束,喜欢就下载吧,查找使用更方便

如果您无法下载资料,请参考说明:

1、部分资料下载需要金币,请确保您的账户上有足够的金币

2、已购买过的文档,再次下载不重复扣费

3、资料包下载后请先用软件解压,在使用对应软件打开

BDNF修饰骨髓间充质干细胞治疗AD的实验研究的开题报告 Title:BDNF-modifiedbonemarrowmesenchymalstemcelltherapyforAlzheimer’sdisease:Anexperimentalstudy Introduction: Alzheimer’sdisease(AD)isaneurodegenerativedisordercharacterizedbytheprogressivelossofcognitivefunctionandmemory.Currently,thereisnocureforAD.However,stemcelltherapyhasemergedasapromisingtreatmentoptionforthisconditionduetothepotentialofstemcellstorepairdamagedtissues.Bonemarrowmesenchymalstemcells(BMSCs)areparticularlypromising,astheycandifferentiateintoavarietyofcelltypesandareeasilyaccessiblefortransplantation.Furthermore,brain-derivedneurotrophicfactor(BDNF)hasbeenshowntoimprovecognitivefunctioninADpatients.Therefore,theaimofthisstudyistoinvestigatethetherapeuticpotentialofBDNF-modifiedBMSCsinthetreatmentofAD. ResearchQuestion: CanBDNF-modifiedBMSCtherapyeffectivelyimprovecognitivefunctioninADrats? Hypothesis: BDNF-modifiedBMSCtherapywillimprovecognitivefunctioninADratscomparedtonon-modifiedBMSCtherapyornotreatment. Methodology: ThestudywillusearatmodelofADinducedbytheadministrationofamyloid-βpeptide.BMSCswillbeisolatedfromthebonemarrowoftheratsandculturedinvitro.TheBMSCswillthenbetransfectedwithaBDNF-expressinglentivirus(experimentalgroup)oracontrollentivirus(controlgroup).Theratswillbedividedintothreegroups:anexperimentalgroupreceivingBDNF-modifiedBMSCs,acontrolgroupreceivingnon-modifiedBMSCs,andashamgroupreceivingnotreatment.CognitivefunctionwillbeassessedusingtheMorriswatermazetest,andtheeffectsonneuronsurvivalandsynapticplasticitywillbeassessedusingimmunohistochemistry,Westernblotting,andelectronmicroscopy. Anticipatedresults: ItisanticipatedthatBDNF-modifiedBMSCtherapywillresultinsignificantimprovementsincognitivefunctioncomparedtonon-modifiedBMSCtherapyornotreatment.Additionally,theremaybeanincreaseinneuronsurvivalandsynapticplasticityinthebrainsofratsreceivingBDNF-modifiedBMSCs.