酒糟单细胞蛋白饲料生产技术研究.pdf

第27卷第4期湖南农业大学学报(自然科学版)Vol.27No.4
2001年8月JournalofHunanAgriculturalUniversity(NaturalSciences)Aug.2001

文章编号:1007-1032(2001)04-0317-04
酒糟单细胞蛋白饲料生产技术研究

卢向阳1,饶力群1,彭丽莎2,向华1,鲁小春3,罗泽民1
(1.湖南农业大学理学院,湖南长沙410128;2.湖南农业大学植物科学技术学院,湖南长沙410128;3.湖南农
业大学食品科学技术学院,湖南长沙410128)
摘要:为了充分利用酒糟资源,减轻酒糟对环境的污染,以优良菌种117号产朊假丝酵母、136号热带假丝酵母、
白地霉和扣囊拟内孢霉混合发酵酒糟,生产酒糟单细胞蛋白饲料.结果表明:发酵最适起始pH为5.0左右;适宜的
发酵培养基配方为80%酒糟,15%麦麸,3%玉米粉,1%豆饼,1%尿素,0.05%磷酸二氢钾,发酵前用适量糖化酶预
处理酒糟能明显提高酒糟蛋白质含量,通过扩大发酵试验,100g酒糟中粗蛋白含量由18.1g增加到28.6g,人和
成年哺乳动物必需氨基酸及钙、磷含量显著增加,粗纤维和单宁含量明显减少,酒糟单细胞蛋白饲料可作为配合饲
料的新型优质蛋白源;采用分割留种培养能生产高质量、低成本的酒糟单细胞蛋白饲料.
关键词:酒糟;单细胞蛋白;酵母菌;饲料;发酵
中图分类号:TS261.9文献标识码:A

ProductionofSingle-CellProteinFeedfromWholeSpillage

LUXiang-yang1,RAOLi-qun1,PENGLi-sha2,XIANGHua1,LUXiao-chun3,LUOZe-min1
(1.CollegeofSciences,HNAU,Changsha410128,PRC;2.CollegeofPlantScienceandTechnology,HNAU,
Changsha410128,PRC;3.CollegeofFoodScienceandTechnology,HNAU,Changsha410128,PRC)
Abstract:Inordertomakefulluseofwholestillageandtoalleviatethepollutionofwholestillage
onenvironment,experimentsonproductionofsingle-cellprotein(SCP)feedbyfermentingwhole
stillagewithfourkindsofexcellentmicroorganisms(includingCandidautilis117,Candida
tropicalis136,GeotrichumcandiumlinkandEndomycopsissp.)wereconducted.Theresults
showthat:
1)TheoptimuminitialpHoffermentionisaboutpH5.0;
2)Thefavourableculturecompositionoffermentationincludes80%ofwholestillage,15%of
wheatbran,3%ofcornmeal,1%ofsoya-beencake,1%ofureaand0.05%ofKH2PO4;
3)TheproteincontentsofSCPfeedfromwholestillageincreaseobviouslybyhydrolyzing
wholestillagewithglucoamylasebeforefermentation;
4)Afterfermentation,theproteincontentsoftheproductincreasesto28.6g/100gDW,and
itis1.58timesofthewholestillage;thecontentsofessentialaminoacids,calciumand
phosphorusincreaseobviously,whilethecontentsoffiberandtanindecreaseinalargescale.
Therefore,thefermentationproductcanbeusedasanewkindofexcellentproteinsourcein
makingcompoundfeed.
5)HighqualityandlowcostSCPfeedfromwholestilagecanbeproducedbyinoculating12%
offermentationproduc