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DNAfingerprinting(DNA指纹图谱).ppt

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DNAfingerprinting (DNA指紋圖譜)HistoricalbackgroundWhatisDNAFingerprinting?ThedifferentsequenceDNAFingerprintingusingVNTR'sMakingDNAFingerprints3:TransferofDNAtonylon.ThedistributionofDNApieces istransferredtoanylonsheet byplacingthesheetonthegel andsoakingthemovernight. 4-5:Probing.AddingradioactiveorcoloredprobestothenylonsheetproducesapatterncalledtheDNAfingerprint.4-6:DNAfingerprint. ThefinalDNAfingerprintisbuiltbyusingseveralprobes(5-10ormore)simultaneously.PracticalApplicationsofDNAFingerprinting2.CriminalIdentificationandForensics3.PersonalIdentification5.DevelopingCuresforInheritedDisordersConsiderationswhenevaluatingDNAevidence2.ProblemswithDeterminingProbabilityB.TechnicalDifficultiesWhenevaluatingaDNAmatch,thefollowingquestionsshouldbeasked: -Coulditbeanaccidentalrandommatch? -Ifnot,couldtheDNAsamplehavebeenplanted? -Ifnot,didtheaccusedleavetheDNAsampleattheexacttimeofthecrime? -Ifyes,doesthatmeanthattheaccusedisguiltyofthecrime?