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色谱柱和色谱系统的故障.ppt

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HPLCColumnandSystemTroubleshooting色谱柱和色谱系统的故障检修HPLCComponentsHPLC的组成CategoriesofColumnProblems色谱柱问题的类型PressureIssues压力问题DeterminingtheCauseandCorrectingHighBackPressure查出原因纠正压力ColumnCleaning柱子清洗ColumnCleaning柱子清洗HowtoChangeaFrit怎样更换滤头PreventingBackPressureProblems压力问题的预防PreventingBackPressureProblems:In-LineDevices压力问题的预防:在线装置PreventingBackPressureProblems:SamplePreparation压力问题的预防:样品制备PeakShapeIssues峰形问题SplitPeaks裂峰SplitPeaks裂峰ColumnContamination柱污染SplitPeaks裂峰InjectionSolventEffects溶剂化效应DeterminingtheCauseofSplitPeaks裂峰原因的确定PeakTailing,BroadeningandLossofEfficiency峰拖尾、变宽和柱效降低PeakTailingColumn“SecondaryInteractions”峰拖尾柱“次级效应”PeakTailingColumn“SecondaryInteractions”峰拖尾柱“次级效应”PeakTailing峰拖尾ColumnContamination柱污染PeakTailing/BroadeningSampleLoadEffects峰拖尾/变宽样品过载效应PeakBroadening,SplittingColumnVoid峰变宽,裂开柱塌陷BroadPeaksUnknown“Phantom”PeaksPeakTailing峰拖尾InjectorSealFailure进样器密封性差PeakTailing峰拖尾Extra-ColumnVolume柱外死体积效应DeterminingtheCauseofPeakTailing峰拖尾原因的确定RetentionIssues保留时间问题ChangesinRetentionSameColumn,OverTime相同柱子上保留时间改变,超时MobilephaseChangeCausesChangeinRetention流动相改变引起保留时间改变ColumnAging/EquilibrationCausesRetention/SelectivityChanges柱老化／平衡不够引起保留时间／选择性改变DeterminingtheCauseofRetentionChangesonthesameColumn同一柱上保留时间改变原因的确定ChangeinRetention/SelectivityColumn-toColumn柱与柱之间保留时间/选择性的改变Lot-to-LotSelectivityChange(pH)批与批之间选择性改变(pH)Conclusions结论