土霉素的生产工艺.doc

天津渤海职业技术学院2012届毕业生毕业论文

土霉素的生产工艺

目的：土霉素生产工艺的概述。

摘要：土霉素提取工艺是通过黄血盐-硫酸锌作净化剂协同去除

蛋白质等高分子杂质，然后用122-2树脂脱色进一步净化土霉素滤液，

最后调pH至4.8左右结晶得到土霉素碱产品。

关键词：生产工艺；土霉素；黄血盐-硫酸锌

目录
1文献综述·················································4
1.1霉素简介·····················································5
1.1.1名称与化学结构式············································5
1.1.2性状与理化质················································6
1.2土霉素生产菌种···············································6

2土霉素生产工艺·······················7
土霉素的生产工艺流程概述····································7
2.2发酵工艺流程················································7
2.2.1斜面孢子制备················································7
2.2.2一级种子罐发酵··············································7
2.2.3二级种子罐发酵··············································7
2.2.4三级发酵罐发酵··············································8
2.3酸化过滤工艺流程············································9
2.3.1酸化························································9
2.3.1.2发酵液的纯化···············································10
2.3.2过滤·······················································10
2.4脱色结晶工艺流程············································11
2.4.1脱色························································11
2.4.2结晶························································11
2.5.1离心·······················································13
2.5.2干燥·······················································13
2.5.2.1湿成品干燥记录·············································13

3土霉素生产的废水处理····················13
3.2该生产废水处理方案··········································14
3.2.1高压脉冲电凝水处理技术进行预处理····························14
3.2.2复合絮凝(气浮)技术降低抗生素残余效价························15
3.2.3组合兼氧技术进行水解酸化····································15
3.2.4生物活性炭滤池进行深度处理··································16
3.3生产废水处理设备及参数······································17

4土霉素的微生物限度检验方法···························18
4.1材料························································18
4.2方法与结果···········