1仿生脉冲电磁场对去卵巢大鼠骨质疏松治疗作用及机理.pdf

869重庆医学2005年7月第34卷第7期

·论著·
仿生脉冲电磁场对去卵巢大鼠骨质疏松治疗作用及机理的初步探讨

谢肇1,李起鸿1,许建中1,孟萍2,谭祖键1
(第三军医大学西南医院:1.骨科,全军矫形外科中心;2.老年科,重庆400038)

摘要:目的观察仿生电磁场对去卵巢大鼠骨质疏松的治疗作用,探讨其作用机理。方法6月龄雌性未孕Wistar大鼠
40只,按体质量随机分为模型组(OVX)、假手术组(Sham)、仿生电磁场治疗组(EM)、雌激素治疗组(E)。OVX、EM、E组行双侧
卵巢切除术,Sham组行假手术。术后8周,E组苯甲酸雌二醇肌肉注射,0.5mg/kg,1次/2周。EM组大鼠暴露于仿生电磁场治
疗,1h·1次-1·d-1,OVX、Sham组不予以任何处理,作为对照组。治疗10周后测量腰椎骨密度、椎体的最大载荷、右侧胫骨骨
结构以及血尿生化。结果仿生电磁场显著改善了大鼠腰椎骨密度(P<0.01)、最大载荷(P<0.01),骨结构也明显改善。血尿
生化显示骨形成大于骨吸收。结论仿生电磁场对去卵巢大鼠骨质疏松具有明显的治疗作用,增强成骨细胞功能,抑制破骨细胞
功能是其治疗作用的可能机理之一。
关键词:仿生电磁场;绝经后骨质疏松症;治疗作用;机理
中图分类号:R365.68文献标识码:A文章编号:167128348(2005)0720968203

ThetherapeueticeffectofbionicselectromagneticfieldsinOVX2inducedosteoporosisratsanditsmechanism
XIEZhao,LIQi2hong,XUJian2zhong,etal.
(DepartmentofOrthopedics,OrthopedicCenterofPLA,SouthwestHospital,
theThirdMilitaryMedicalUniversity,Chongqing400038,China)
Abstract:ObjectiveToinvestigatethetherapeueticeffectofbionicselectromagneticfieldsandtoexplorethemechanismof
thatinpostmenopausalosteoporosis.MethodsThefourty62montholdfemaleWistarratswererandomlydividedintofourdifferent
groups:ovariectomygroup(OVX),shamoperationgroup(Sham),Egroup(Estrogen+OVX)andEMgroup(BEMF+OVX).
Allratsweresubjectedtobilateralovariectomyexceptshamoperationgroup.After8weeksoperation,theEgroupratsweregiven
estrogen0.5mg/kg,1/2W.EMgroupratswereexposedtobionicselectromagneticfields,1h/d.OVXandShamgroupratswere
givennothing.Afterthetreatmentsfinished,thespecimensoflefttibia.ResultsAfterthetreatmentwithBEMF,BMDatthe
lumbarspinesignificantlyincreasedby24.3%(vs.OVX),themaximumloadofEMgroupmarkedlysignificantlyincreasedby
22%(vs.OVX)(P<0.01).Thetrabecularvolumeandtrabecularconnectionssignificantlyincreased(vs.OVX).TheTRAP
concentrationsinEMgroupdecreased(vs.OVX)(P<0.05),TheBGPconcentrations,theALPconcentrationsincreased(vs.
OVX)(P<0.05).ConclusionTheBEMFareusefulinthetreatmentofpostmenopausalosteoporosisresultingfromovariectomy.
BuildinguptheosteoblastsandcontrollingtheosteoclastsmaybeoneofmechanismsoftherapeuticeffectofBEMFo