槲皮素对NIH_3T3细胞氧化损伤的保护作用_中医中药_医药.pdf

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(基础研究(
槲皮素对7R<F%@%细胞氧化损伤的保护作用
宫璀璀"郑玉霞"曹阳"郑乃刚#李璇"刘力源"吴景兰$
%%9M%医院济南军区检验中心!河南郑州$M""$!$
$郑州大学分子细胞生物学研究中心!河南郑州$M""M!&

8摘要?目的探讨槲皮素对7R<F%@%细胞氧化损伤的保护作用"方法取处于对数生长期的%@%细
胞!平均分为以下四个实验组"槲皮素前保护组%L9组&’先加含有M"!034NO槲皮素培养液培养!$/!再
换含有的培养液培养槲皮素后保护组组先加含有的培养
"SM0034NO<!=!%"0DP";L!>’"SM0034NO<!=!
液培养再换含有槲皮素培养液培养损伤组组先加含有
%"0DP$M"!034NO!$/$<!=!;<!>’"SM0034NO<!=!
的培养液培养%"0DP!再换仅含有9"K的胎牛血清的培养液培养!$/"对照组;H组>’仅加9"K胎牛血清的
A+B+培养液培养!$/"收集并裂解各组细胞!检测细胞内@FG=H#C=A#IC<F&T#IC<#7=C#7=#+AG
的变化!应用+@@实验检测%@%细胞的生存率"结果L9与L!#<!组相比!L9组细胞内@FG=H#C=A#
IC<F&T#IC<均有增加!7=C#7=无明显改变!+AG减少!细胞存活率明显增加"结论槲皮素可能是通
过上调抗氧化酶系活性对%@%细胞的氧化损伤产生保护作用"
关键词细胞裂解抗氧化损伤槲皮素
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8中图分类号?U!V!1W98文献标识码?G

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