成人骨髓间充质干细胞向甲状腺细胞的诱导分化.pdf

中国组织工程研究与临床康复第15卷第6期2011–02–05出版
JournalofClinicalRehabilitativeTissueEngineeringResearchFebruary5,2011Vol.15,No.6

成人骨髓间充质干细胞向甲状腺细胞的诱导分化

张勤，刘东源

Differentiationofadultbonemarrowmesenchymalstemcellsintothyroidcells

ZhangQin,LiuDong-yuan

Abstract

BACKGROUND:Thyrotrophin,insulinorinsulin-likegrowthfactorplayimportantrolesindevelopment,differentiation,Departmentof
heterogenicexpressionandfunctionproductionofthyroidcells.Thus,thisstudysimulatedthyroidinvivoembryogenesis,andGeneralSurgery,the
401Hospitalof
graduallyaddedabove-mentionedstimulatingfactortofurtherinducebonemarrowmesenchymalstemcells(BMSCs).ChinesePLA,
OBJECTIVE:ToinvestigatethefeasibilityofdifferentiationoftheadultBMSCsintothyroidcellsunderspecificcircumstancesinQingdao266000,
vitro.ShandongProvince,
METHODS:BMSCswereisolatedbythedensitygradientmethod.BMSCswereinducedwiththyrotrophinandinsulin.China
Morphologicalchangeswereobservedbytheinvertedopticalmicroscopeandtransmissionelectronmicroscopy.Immune
ZhangQin,Associate
fluorescencedetectionmethodwasusedtodifferentiateadultBMSCs.chiefphysician,
RESULTSANDCONCLUSION:Onday3followingcultureintheinductiongroup,BMSCmorphologychangedfromspindletoDepartmentof
round,ellipseortriangle,grewirregularly,withclearboundary.Onday8,adherentembryobecameflat.Onday7,differentiatedGeneralSurgery,the
cellsexpressedthyroidcellgeneTSHr.Onday9,differentiatedcellmarkersTTF-1expressionwasdetectedinthyroidcells.401Hospitalof
ResultssuggestedthatBMSCscanbeinducedtodifferentiateintothyroidcellsinvitroandareidealseedcellsinthestudyofChinesePLA,
Qingdao266000,
tissueengineeringtreatmentofthyroiddiseases.
ShandongProvince,
China
ZhangQ,LiuDY.Differentiationofadultbonemarrowmesenchymalstemcellsintothyroidcells.ZhongguoZuzhiGongchengzhangqin401@
YanjiuyuLinchuangKangfu.2011;15(6):951-954.[http://www.crter.cnhttp://en.zglckf.com]126.com

Correspondenceto:
LiuDong-yuan,
摘要
Departmentof
GeneralSurgery,the
背景：促甲状腺素、胰岛素或类胰岛素生长因子等对甲状腺细胞的发育分化、特异基因的表达及功能的产生等起着重要作401Hospitalof
用，故实验模拟甲状腺的体内胚胎发生