中轻度过量补碘对非碘缺乏大鼠甲状腺功能和形态的影响.pdf

2003年l0月第42卷第l0期—ChinJInternMed,October2003,Vo142yNo．10·705·
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．论著

中轻度过量补碘对非碘缺乏大鼠甲状腺
功能和形态的影响

高天舒胡凤楠滕卫平

【摘要】目的研究中轻度过量补碘对非碘缺乏Wistar大鼠甲状腺功能和形态的影响。方法
将Wistar大鼠随机分为双蒸馏水(DDW)、3倍碘、6倍碘、10倍碘、20倍碘5组，每组10只。固相免
疫放射分析法(IRMA)测大鼠血清促甲状腺激素(TSH)，放射免疫分析法(RIA)测血清总T(Tr4)、
总T(Tr)、反T(rT)和甲状腺内Tr4。砷铈分光光度法测定尿碘。光、电镜下观察，图像分析仪测
量滤泡上皮细胞高度和滤泡腔面积。结果与DDW组比较，补充3倍以上碘各组90d时血清TSH
值增高，但差异无显著性；血清Tr1明显降低(P=0．0001)，Tr4明显增高(P=0．001)，组织Tr4也明
显增高(P=0．0001)，血清rT1值无明显差异；滤泡腔面积增大，上皮细胞变扁，胞核染色深，滤泡融
合破裂，巨滤泡形成，毛细血管减少；上皮细胞内质网扩张，次级溶酶体增多，微绒毛减少，染色体浓
集。结论补充3倍以上碘使非碘缺乏大鼠出现甲状腺功能减退倾向，抑制和破坏大部分甲状腺滤
泡上皮细胞。
【关键词】碘；甲状腺

Efectofmildandmoderateexcessiveiodinesupplementatlononthyroidfunctionandmorphologyin
non-iodinedeficiencyratmodelGAOTian-shu，日Feng-nan，TENGWei-ping．Departmentof
Endocrinology，FirstAffiliatedHospital，ChinaMedicalUniversity，Shenyang110001，China
IAbstract】objectiveTostudytheefectofmoderateandmildiodineexcessonthyroidfunotion
andmorphologyinnoniodinedeftciencyWistarrats．MethodsSerumthyroid．stimulatinghormone(rSH)
wasmeasuredwithsolidphaseradioimmunoassay(IRMA)andserumtotalT4(Tr4)，Tr3，reverseT3(rT3)
andthyroidTLwithradioimmunoassay(RIA)．Cer．Arsenitecolormetricmethodwasusedtomeasure
medianurinaryiodine(MUI)．Thyroidmorphologicalchangeswereobservedunderopticalandelectronic
microscope．Metamorphicimagingsystemwasusedtomeasuretheheightofthyrocytesandtheareasof
thyroidf0l1iculatcavities．ResultsIodineintakeofthreetimesthannormalfor90dayscouldincrease
serumTSHbutwithoutsignificantdiference；SerumrlT1valuesweremarkedlylowerthanthoseincontro1
．
P=0．0001．SerumandthyroidTLvaluesweremarkedlyhigherthanthoseindoubledistilledwater
(DDW)group，Pvaluebeing0．001and0．0001respectively．However．serumrT3didnotchange
markedlywhencomparedwiththatinDDWgroup．Theareaoffolicularcavityincreasedfolliculatcels
．
becamefiat，nucleusstaineddarker，f0uiclesbrokeandcombined，giantfollicleswereformed．andthe
capiUariesaroundthefoiliculeswerereduced．，I1heheightofthyrocyteswasmarkedlylowerthanthosein
DDWgroup，whiletheareasofthyroidfollicularcavitieswerema