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坐骨神经损伤大鼠模型鞘内移植神经干细胞后脊髓背角和.pdf

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中国组织工程研究第16卷第10期2012–03–04出版 ChineseJournalofTissueEngineeringResearchMarch4,2012Vol.16,No.10 坐骨神经损伤大鼠模型鞘内移植神经干细胞后脊髓背角和背根神经节 ★ 脑源性神经营养因子的表达 张民皓1，胡益民1，张红2，胡玉萍1，刘清珍1，王洪超2，李伟彦1 Brain-derivedneurotrophicfactorexpressioninthespinaldorsalhornanddorsalrootganglion inaratmodelofsciaticnerveinjuryafterintrathecaltransplantationofneuralstemcells ZhangMin-hao1,HuYi-min1,ZhangHong2,HuYu-ping1,LiuQing-zhen1,WangHong-chao2,LiWei-yan1 Abstract BACKGROUND:Themodelofsciaticnerveinjurycanbeusedtotestthehyperalgesiaandparaesthesiacausedbynocuous1Departmentof heatstimulationandmechanicalstimulation.Anesthesiology, NanjingGeneral OBJECTIVE:Toinvestigatetheexpressionofbrain-derivedneurotrophicfactor(BDNF)inthespinaldorsalhornanddorsalroot HospitalofNanjing ganglioninaratmodelofsciaticnerveinjuryafterintrathecaltransplantationofneuralstemcells.MilitaryCommand, METHODS:Seventy-twoadultSprague-Dawleyratswererandomlydividedintosham-operation,controlandexperimentalPLA,Nanjing groups.Ratmodelofsciaticnerveinjurywasestablishedinthecontrolandexperimentalgroups.Inthesham-operationgroup,210002,Jiangsu Province,China; onlysciaticnervewasexposed,withoutligation.Intrathealtransplantationwasperformedat3and10daysafterinductionof2 sciaticnerveinjury.30μLofneuralstemcellsuspensionwasinjectedintotheexperimentalgroup,and30μLcellculturesolutionKeyLaboratoryof Anesthesiologyof wasinjectedintothesham-operationandcontrolgroups.JiangsuProvince, RESULTSANDCONCLUSION:Comparedwithsham-operationgroup,mechanicalwithdrawalthresholdandthermalwithdrawalXuzhouMedical latencyweregraduallyreducedat3daysaftertransplantation,decreasedtothelowestlevelat7days(P<0.01)andrecoveredCollege,Xuzhou topre-transplantationlevelat21daysinthecontrolandexperimentalgroups.At7and14daysaftertransplantation,mechanical221002,Jiangsu withdrawalthresholdandthermalwithdrawallatencyweresignificantlyincreasedintheexperimentalgroupthaninthecontrolProvince,China group(P<0.01).Comparedwithcontrolgroup,at7,14and21daysaftertransplantation,BDNFe