冠心合剂对心梗后大鼠VEGF表达的影响_祝光礼.pdf

第29卷第2期中华中医药学刊Vo.l29No.2
2011年2月CHINESEARCHIVESOFTRADITIONALCHINESEMEDICINEFeb.2011

冠心合剂对心梗后大鼠VEGF表达的影响

祝光礼,钱宝庆,周凡,刘昭
(浙江中医药大学附属广兴医院心内科,浙江杭州310007)

摘要:目的:观察冠心合剂对急性心肌梗死(AMI)大鼠血管内皮生长因子(Vascularendothelialgrowthfac-
tor,VEGF)表达的影响。方法:结扎冠状动脉左前降支法建立大鼠急性心肌梗死模型,观察冠心合剂对心梗后大
鼠血清VEGF浓度、心肌VEGF蛋白表达及梗死面积的影响。结果:冠心合剂治疗组及阳性对照组血清VEGF浓
度及心肌VEGF蛋白的表达增加,高于模型组和假手术组(P<0.05);随冠心合剂剂量的增加,梗死面积逐渐减
小;冠心合剂大剂量组疗效与西药对照组无明显差异(P>0.05)。结论:冠心合剂可能通过促进心肌梗死大鼠心
肌VEGF蛋白表达、分泌,从而促进梗死心肌血管新生。
关键词:冠心合剂;心肌梗死;血管内皮生长因子;促血管新生
中图分类号:R285.5文献标识码:A文章编号:1673-7717(2011)02-0247-03

EffectsofGuanxinDecoctionontheExpressionofVEGFin
RatofAcuteMyocardialInfarction

ZHUGuang-l,iQIANBao-qing,ZHOUFan,LIUZhao
(DepartmentofCardiology,GuangxingHospitalAffiliatedtoZhejiangChineseMedicalUniversity,Hangzhou310007,Zhejiang,China)

Abstrac:tObjective:ToinvestigatetheeffectofGuanxinDecoctiononmyocardialangiogenesisandtheexpressionof
VEGFinratswithacutemyocardialinfarction.Methods:Leftanteriordescendingcoronaryarteries(LAD)havebeenliga-
tedinSDratsafteranesthetizedby2.5%pentobarbitalsodium.ThelevelofVEGFinbloodserumwasmeasured2weeks
aftertheexperimentbyELISArespectively.Theheartspecimenwerefixed,sectionedandcolorizedbymeansofHEsta-i
ningandimmunohistochemicalstainingtoobserveVEGFthroughmicroscope.Results:GuanxinDecoction-treatment
groupandpositivecontrolgroupwerehigherthanthemodelgroupandthesham-operationgroupintheexpressionof
VEGFprotein(P<0.05).ThelevelofVEGFinbloodserumincreasedcorrespondinglywiththeincreaseofthedoseof
GuanxinDecoction,whilethemyocardialinfarctionscopereducing.Thecurativeeffectofthehigh-dosageGuanxinDe-
coctionissimilarwithbFGFandheparin(P>0.05).Conclusion:ThemechanismofAMIratangiogenesiswhichispro-
motedbyGuanxinDecoctionmaybeassociatedwiththeincreaseofexpressionandexcretionofVEGFincardiacmyo-
cytes.
Keywords:GuanxinDecoction;AcuteMyocardialInfarction;VascularEndothelialGrowthFactor;TherapeuticAn-
giogenesis

冠心合剂由黄芪合用失笑散而成,具有补益心脾之1材料与方法
气,活化心胸之瘀滞的功效。经我们长期临床观察,应用1.1实验动物清洁级雄性SD大鼠65只,体重(200?
黄芪失笑散治疗冠心病心肌缺血患者,可有效缓解胸