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I型鸭肝炎病毒中国分离株的全基因组序列测定与分析.pdf

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VIROLOGICASINICA,October2007,22(5):353-359 CLCnumber:S852.65Documentcode:AArticleID:1674-0769(2007)05-0353-07 CompleteGenomicSequenceofaChineseIsolateofDuck HepatitisVirus* Guang-qingLIU1**,FeiWANG2,ZhengNI1,TaoYUN1,BinYU1, Jiong-gangHUANG1andJian-PingCHEN** (1.InstituteofVirologyandBiotechnology,ZhejiangAcademyofAgriculturalSciences,Hangzhou310021, China;2.CollegeofAnimalScienceandTechnology,AnhuiAgricultureUniversity,Hefei230036,China) Abstract:ThecompletegenomicsequenceofDuckhepatitisvirus1(DHV-1)ZJ-Visolatewas sequencedanddeterminedtobe7691nucleotides(nt)inlengthwitha5′-terminalun-translatedregion (UTR)of626ntanda3′-terminalUTRof315nt(notincludingthepoly(A)tail).Onelargeopen readingframe(ORF)wasfoundwithinthegenome(nt627to7373)codingforapolypeptideof2249 aminoacids.Ourdataalsoshowedthatthepoly(A)tailofDHV-1hasatleast22A’s.Sequence comparisonrevealedsignificanthomology(from91.9%to95.7%)betweentheproteinsequencesofthe ZJ-Visolateandthoseof21referenceisolates.AlthoughDHV-1hasbeenclassifiedasanunassigned virusinthePicornaviridaefamily,itsgenomeshowedsomeuniquecharacteristics.DHV-1contains3 copiesofthe2Ageneandonly1copyofthe3Bgene,andits3′-NCRislongerthanthoseofother picornaviruses.PhylogeneticanalysistodosequencehomologybasedontheVP1proteinsequences showedthattheZJ-VisolateshareshighsequencehomologywiththereportedDHV-1isolates(from 92.9%to99.2%),indicatingthatDHV-1isgeneticallystable. Keywords:Duckhepatitisvirus1(DHV1);Phylogenetictree;vp1gene Duckhepatitisvirus(DHV)causesahighlyhavebeendescribedandclassifiedaspicornaviruses contagiousdiseaseinyoungduckswhichoften(4,11).Noantigenicrelationshipsexistamongthe3 associatedwithlivernecrosis,hemorrhagesandhighserotypes(8,14),ofwhichDHV-1isdistributed mortality.ThediseasewasfirstdescribedinLongworldwideandthushasthemosteconomicrelevance. Islandin1949(7).Subsequently,outbreakswereDHV-1wasoriginallyclassifiedasanenterovirus(13). reportedfromEngland,Canada,Germany,JapanandLateron,itwasfoundtobemorecloselyrelatedtothe els