甘蓝型油菜遗传图谱构建与无花瓣性状QTL定位.pdf

作物学报ACTAAGRONOMICASINICA2007,33(8):1246-1254http:PPwww.chinacrops.orgPzwxbP
ISSN049623490;CODENTSHPA9E2mail:[e-mail]

甘蓝型油菜遗传图谱构建与无花瓣性状QTL定位
张洁夫1,2戚存扣2栗根义3浦惠明2陈松2陈新军2高建芹2陈锋2
顾慧2傅寿仲2,3X
(1南京农业大学作物遗传与种质创新国家重点实验室,江苏南京210095;2江苏省农业科学院经济作物研究所,江苏南京210014;3Departmentof
PlantScience,UniversityofManitoba,WinnipegR3T2N2,Canada)

摘要:以无花瓣油菜APL01与正常有花瓣品种M083杂交的BC1F1为基础群体,利用RAPD、SSR和SRAP技术获得251
个分子标记,包括219个SRAP、25个SSR和7个RAPD标记,构建了由19个连锁群组成的分子标记遗传图谱,根据共同
的分子标记,建立该图谱与甘蓝型油菜高密度图谱的对应关系。利用WinQTLCart210软件对无花瓣性状进行QTL扫描,
获得4个与无花瓣性状相关的QTL,QAP5位于N5连锁群的A0226Bb152～m31e40b区间,解释花瓣度表型变异的3.71%;
QAP6位于N6连锁群的m25e7～OPY9区间,解释花瓣度表型变异的3.02%;QAP8位于N8连锁群的A0226Gb468～
m29e20区间,解释花瓣度表型变异的30194%;QAP15位于N15连锁群的m21e4b～A0225Bb201区间,解释花瓣度表型变
异的21196%。QAP8和QAP15为2个主效QTL,可用于无花瓣性状的标记辅助选择,QAP5和QAP6为修饰基因位点。

关键词:油菜;遗传图谱;无花瓣;QTL

GeneticMapConstructionandApetalousnessQTLsIdentificationinRapeseed
(BrassicanapusL.)

ZHANGJie2Fu1,2,QICun2Kou2,LIGen2Yi3,PUHui2Ming2,CHENSong2,CHENXin2Jun2,GAOJian2Qin2,CHENFeng2,

GUHui2,andFUShou2Zhong2,3

(1NationalKeyLaboratoryofCropGeneticsandGermplasmInnovation,NanjingAgriculturalUniversity,Nanjing210095,Jiangsu;2InstituteofIndustrialCrops,
JiangsuAcademyofAgriculturalSciences,Nanjing210014,Jiangsu,China;3DepartmentofPlantScience,UniversityofManitoba,WinnipegR3T2N2,Canada)
Abstract:Apetalousrapeseedhastheadvantagesofhighsolarraditionutilizationefficiency,lowsclerotiniaincidence
ratio,strongrootassimilationefficiency,andhighyieldpotentialowingtothelossofpetal.Apetalousnessiscontrolledby
twotofourrecessivegenesinrapeseed.Allresearchersconsidertheplantswithlessthan10%petalousdegree(PD)asthe
apetalous,butthedistributionofPDinsegregationgenerationisconsecutive.Theapetalousshouldbetreatedasa
quantitativetraittoscreenQTLs.Inpresentresearch,anapetalousrapeseedlineAPL01wascrossedwithanormalpetalous

lineM083todevelopasegregatingpopulationBC1F11Thispopulationwasusedtoconstructageneticmapandidentify
QTLsaffectingtheapetalousinrapeseed(BrassicanapusL.).Totally,219SRAP,25SSR,and7RA