重症急性胰腺炎大鼠肺脏iNOSmRNA表达及丹参的干预效应.pdf

第27卷第15期第三军医大学学报Vol.27,No.15

2005年8月ACTAACADEMIAEMEDICINAEMILITARISTERTIAEAug.20051569

文章编号:100025404(2005)1521569204论著

重症急性胰腺炎大鼠肺脏iNOSmRNA表达及丹参的干预效应

112133
张莹,石承先,别平,任娟娟,李玉祥,黄平(1贵州省人民医院普通外科,贵阳550000;2第三军医大学西
南医院全军肝胆外科研究所,重庆400038;3遵义医学院组织与胚胎学教研室,遵义563003)

提要:目的探讨重症急性胰腺炎(severeacutepancreatitis,SAP)大鼠肺脏诱导型一氧化氮合酶(iNOS)mRNA
的表达及其与肺组织损伤的关系和丹参的治疗效应。方法45只Wistar大鼠随机分为3组。SAP模型组、丹参组均以
5%牛磺胆酸钠经胰管内逆行注射(1ml/kg)复制SAP模型,丹参组则在制模后立即肌肉注射丹参(2ml·kg-1·d-1),
每6h1次,共给药4次。对照组仅行假手术。各组动物在术后24h测血一氧化氮(NO)、淀粉酶(AMY)和腹水量。使
用原位杂交和图像分析检测肺iNOSmRNA的表达强度,并观察其病理变化。结果AP模型组血中NO、AMY、腹水量
和肺脏iNOSmRNA表达显著高于丹参组和对照组(P<0105,P<0101);丹参组病理改变较模型组减轻。结论SAP
时肺组织iNOSmRNA高表达导致NO过度生成并与肺脏损伤有关;丹参能抑制肺组织iNOSmRNA的过度表达,从而对
肺组织起保护作用。
关键词:重症急性胰腺炎;肺脏;iNOSmRNA;丹参;保护
中图法分类号:R282.71;R563;R576.02文献标识码:A

EffectofsalviamiltiorrhizaoninduciblenitricoxidesynthasemRNAexpressionin
lungsofratswithsevereacutepancreatitis
112133
ZHANGYing,SHICheng2xian,BIEPing,RENJuan2juan,LIYu2xiang,HUANGPing(1DepartmentofGeneralSur2
gery,GuizhouPeople’sHospital,Guiyang550000,2InstituteofHepatobiliarySurgery,SouthwestHospital,ThirdMilitaryMedicalUniver2
sity,Chongqing400038,3DepartmentofHistologyandEmbryology,ZunyiMedicalCollege,Zunyi563003,China)

Abstract:ObjectiveToinvestigatetheexpressionofinduciblenitricoxidesynthase(iNOS)mRNAin
lungsandtheeffectofsalviamiltiorrhizaonlunginjuryinratswithsevereacutepancreatitis(SAP).Methods
Atotalof45Wistarratswererandomlydividedinto3groups:SAPmodelgroup(SP,n=15),salviamilti2
orrhizagroup(SM,n=15)andshamoperationgroup(SO,n=15).Severeacutepancreatitiswasinducedby
intraductalinjectionof5%sodiumtaurocholate(110ml/kg)inSPgroupandSMgroup.Shamoperationwas
onlymadeinSOgroup.Salviamiltiorrhiza(2ml·kg-1·d-1)wasintramuscularlyinjectedimmediatelyafter
SAPwasinducedinSMgroup,andthesamedosewasadministratedevery6h.Thesamevolumeofphysiologi2
calsalinewasadministratedinSPgroupandSOgroup.Thevolumeofascitesandserumnitricoxide(NO)and
amylase(AMY)weredetermined.iNOSmRNAexpressioninlungwereexaminedbyinsituhybridization.The
patholog