白肋烟分子标记遗传图谱的构建及部分性状的遗传剖析.pdf

作物学报ACTAAGRONOMICASINICA2009,35(9):1646−1654http://www.chinacrops.org/zwxb/
ISSN0496-3490;CODENTSHPA9E-mail:[e-mail]

DOI:10.3724/SP.J.1006.2009.01646

白肋烟分子标记遗传图谱的构建及部分性状的遗传剖析

121211,*
蔡长春柴利广王毅徐芳森张俊杰林国平

1中国烟草白肋烟试验站/湖北省烟草科研所,湖北武汉430030;2华中农业大学作物遗传改良国家重点实验室,湖北武汉430070

摘要:用112个AFLP、6个SRAP标记构建了白肋烟的分子标记遗传连锁图谱,包括22个连锁群(A1~A22),总遗
传长度为1953.6cM,标记的平均间距为20.5cM,有25个标记表现偏分离(17.0%),主要集中在第1、11和14连锁
群上;利用WindowsQTLCartographerVer.2.5软件进行QTL分析,结果表明,共检测到11个主效QTL,其中7个与
化学成分性状相关,另外4个与农艺性状相关。与烟碱、总氮和总糖相关的QTL各2个(btnic1和btnic2)、2个(bttn1
和bttn2)和3个(btts1、btts2和btts3),与株高(btph)、茎围(btls)、节距(btpt)和中部叶长(btl)相关的QTL各1个,没有
检测到与总钾、总叶数和中部叶宽相关的QTL,其中与烟碱和总氮相关的QTL处于共分离状态(分别为btnic1和
bttn1),表明烟叶烟碱合成与氮素代谢之间可能存在某种未知的生物学相关性,11个主效QTL对各性状表型变异的贡
献率在12.3%~26.4%之间。
关键词:白肋烟;遗传连锁图谱;农艺性状;遗传剖析

ConstructionofGeneticLinkageMapofBurleyTobacco(NicotianatabacumL.)
andGeneticDissectionofPartialTraits

CAIChang-Chun1,CHAILi-Guang2,WANGYi1,XUFang-Sen2,ZHANGJun-Jie1,andLINGuo-Ping1,*

1BurleyTobaccoExperimentalStationofChina,HubeiTobaccoResearchInstitute,Wuhan430030,China;2NationalKeyLaboratoryofCrop
GeneticImprovement,HuazhongAgriculturalUniversity,Wuhan430070,China

Abstract:Theagronomictraitsandchemicalcomponentsareveryimportantfactorsaffectingyieldandtobaccoleafquality.Dis-
sectinggeneticcontrolofthesetraitscanfacilitatethebreedingofnewtobaccocultivarsbymarker-assistedselection(MAS).The
objectiveofthisstudywastoidentifyQTLscontrollingpartialagronomictraitsandchemicalcomponentsinburleytobacco.A
doublehaploid(DH)populationofburleytobaccowasusedtoconstructamolecularmarkergeneticlinkagemap.TheDHpopu-
lationwasderivedfromacrossbetweenhighqualitycultivarBurley37withhighnicotinecontentandBurley21withlownico-
tinecontent.ThemappingpopulationwasplantedinmainproductionregionofburleytobaccoinHubeiprovincefortwoyearsto
obtainrepetitivephenotypedata.Onthebasisofthismap,QTLsforfourchemicalcomponentsincludingnicotine(NIC),total
nitrogen(TN),totalsugar(TS),totalpotassium(TK)ofair-curedcentraltobaccoleafandsixagro