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2014!3"&'()*+,March2014 #34$#3%Basic&ClinicalMedicineVol.34No.3 /012:10016325(2014)03033207研究论文 RhoA/Rho7892:;<=4>?@ABCDEFG !"#1,$%1,&'(1,)*+2,),1 (1-.(H4)#I(JKLMH,-.NOP050000;2-.QR4)ST(JKLMH,-.UV056002) 34:*5WXRhoA/Rho789;<=4>?ABCDEFG。67YZ[\]^_`abcdefg (STZ,30mg/kg)hijklm2:;<=4>n:。opLqrst、;<=t_uvwxyzt(uvwx 10mg/(kg·d),L{|hijk)24}~。€;、\、‚ƒ„…8_‚†„…8;Masson‡ˆ_‰Š…‹ €Œ?Ž‘;RTPCR€„C1’“”β1(TGFβ1)_•–t—1’“”(CTGF)mRNA˜™;š›tC €TGFβ1œ˜™;Westernblot€?t—žŸœ‹¡8¢£¤¥¦1‹C(pMYPT1)§¨。89*r st©ª,;<=t4>;、\_„…8«¬­[,?t—4bŽ‘,pMYPT1§¨、TGFβ1*CTGF mRNA˜™«¬®¯(P<001)。*;<=t©ª,uvwxyzt4>„…8°±,?Ž‘X«²³, pMYPT1§¨、TGFβ1*CTGFmRNA˜™«¬´°(P<001)。8:[;7µ¶?t—RhoA/Rho78,Y Z¯·TGFβ1/CTGF˜™,9;<=?ABCD¸¹3ºFG。 ;<=:;<=?ABC;Rho78;„C1’“”β1;•–t—1’“”;uvwx >?@A2:R3643/BCDE:A TherolesofRhoA/Rhokinaseinhepaticfibrosisofratswithtype2diabetes FANGCaixia1,ZHOUHong1,LIGuizhi1,WANGDefeng2,WANGMian1 (1Dept.ofEndocrinology,theSecondHospitalofHebeiMedicalUniversity,Shijiazhuang050000; 2Dept.ofEndocrinology,AffiliatedHospitalofHebeiUniversityofEngineering,Handan056002,China) Abstract:ObjectiveTodeterminewhetherRhoA/Rhokinaseisinvolvedinthepathogenesisofhepaticfibrosisin ratswithtype2diabetes.MethodsAnanimalmodeloftype2diabeteswasdevelopedbyhighfatdietcombined withintraperitonealinjectionoflowdosestreptozotocin(STZ,30mg/kg,i.p.).Atweek24,fastingbloodglu cose,triglycerides,cholesterol,aspartateaminotransferaseandalanineaminotransferaseweremeasured.Thedepo sitionofcollageninliverwasevaluatedbyMassonstainingandthehydroxyprolinedetermination.ThemRNAex pressionsoftransforminggrowthfactor(TGFβ1)andconnectivetissuegrowthfactor(CTGF)inlivertissuewere assessedbyRTPCR.ImmunohistochemistrystainingforTGFβ1wasperformed.Thephosphorylationofmyosin phosphatasetargetsubunit1(pMYPT1)wasmeasuredbyWesternblotanalysis.ResultsComparedwithcontrol rats,bloodglucose,bloodfat,aminotransferaseandthedepositionofcollagenintheliverintheuntreateddiabetic ratsweresignificantlyincreased(P<001);pMYPT1andTGF