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诱导骨髓间充质干细胞向心肌样分化及其移植的可行性.pdf

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中国组织工程研究与临床康复秽73孝彩40筋‘2009—10—01出版 ri JounalofClinicalRehabiltativeTissueEnineerinResearchOctober7209V01．13o．40 gg，0，N 微血管内皮细胞诱导骨髓间充质干细胞向心肌样分化及其移植的可行性★◆ ’’。’。 廖礼强，张晓刚，史若飞，王丁琼，汤为学 Roleofmicrovascularendothelialcellsininducingbonemarrowmesenchymalstemcells diferentiationintocardiomyocytesandimplantationfeasibility ’’。’。 LiaoLi—ianZhanXiao-anShiRuo-eiWanDin—ionTanWei．xue qg，ggg，f，ggqg，g Abstract ’ Departmentof BACKGROUND：Bonemarrowmesenchymalstemcells(BMSCs)haveanenormouspotentialonmyocardium—injureddiseasesCardiologYHrst AffiliateHosital dp． viacardiomyogenesis．however．an}deaIjnducingmethodhasnotbeenfoundatpresent． ChongqingMedical OBJECTIVE：Toinvestiatetheotentialofratmicrovascularendothelialcells(mECs)oninducinBMSCsdiferentiationjnto gpgnvers Uiity． cardiomoctesandtoanalzehefeasibilitoftranslantation． yy，ytypChongqing rv400016．China DESIGN．TIMEANDSETTING：AnobseationaIexperimentbasedoncellsandanimalswasconductedattheChongqingKey； ‘ Chongqing LaboratoryofNeurologybetweenJuly2008andFebruary2009． EmergencyMedical MATERlALS：AtotaIof16clearhealthmaleSraueDawleratsaed2—3monthswereobtainedfrOmtheExerimental ypgygpeneronn Ct，Chgqig AnimaICenter．ChoninMedicaIUniversit． gqgy400016．China； 。 ChoninKe ：rrnsrcaure．s METHODSTranswellcabinof0．4umboewasplacedincultueplatewelItocotuctdoubl~bincultsystemBMSCgqgy Laboratoryof andmECswererespectivelyisolatedandculturedfrom1SpragueDawleyratusingFicolIdensitygradient+adherence Neurolo gy． screeningmethodandexplantmethod．mECswerewelfculturedandreached50％confluency．Themediumwaschangedevery Chongqing r 3das．Followincollectionandfiltration，theconditionedmediumwasobtainedInv#oexerimentwasdividedintodirect1 ygp40006．China contacroubi-chambercoculturerouconditionedmediumrou111andmediumcontrolrouIVand tgp(I)，gp(1I)，gp()gp()， - LiaoLi-iank qg， mocardiaIinductionefectwasobservedintheseconditions．AtotaIof15healthSraueDawleratswerecommittedto yypgy’ Studyingformasters == coronarliationformocardialinfarctionmodelsanddividedintocellimla