人乳腺癌原代培养体外药敏试验研究doc-乳腺癌是女性最常.docx

·论著·人乳腺癌原代培养体外药敏试验研究100021北京中国医学科学院肿瘤医院肿瘤所生物检测中心李艳芬田海梅陈国际2曹冬艳刘毅李茉王小兵刘朝阳梁智程冬婉张伟1[摘要]目的:探讨用ATP生物荧光肿瘤体外药敏检测技术（ATP-TCA）研究乳腺癌药敏的异质性和个体化疗的可行性。方法:用ATP-TCA检测114例乳腺癌标本对13种单药或联合用药的敏感性。结果:个体之间的药物敏感性存在着明显的异质性。单药中最有效的药物为紫杉醇、长春瑞滨、表阿霉素、丝裂霉素和阿霉素分别有73.3％、64.1％、62.3％、49.9％和40.0％的标本对其敏感。联合用药（紫杉醇＋表阿霉素环磷酰胺＋表阿霉素＋氟尿嘧啶）的敏感率高于单药。结论：乳腺癌对抗癌药物的敏感程度存在着异质性。ATP生物荧光肿瘤药敏检测技术可用于为乳腺癌选择合适的化疗药物。[关键词]ATP生物荧光肿瘤药敏检测技术；乳腺癌；化疗药物敏感性中图分类号：R737.9文献标识码：A文章编号：1009-0460(2006)00-0000-00EvaluationofchemosensitivityofhumanbreastcancerdeterminedbyprimarycultureassayinvitroLIYan-fenTIANHai-meiCHENGuo-jiCAODong-yanLIUYiLIMoWANGXiao-bingLIUZhao-yangLIANGZhiCHENGDong-wanZHANGWei.TumorMarkerResearchCenterCancerHospital/InstituteChineseAcademyofMedicalSciencesPekingUnionMedicalCollegeBeijing100021ChinaCorrespondingauther:ZHANGWeiE-mail:[e-mail][Abstract]ObjectiveToinvestigatetheheterogeneityofchemosensitivityinbreastcancerusinganATP-tumorchemosensitivityassay(ATP-TCA)andthefeasibilityofindividualchemotherapy.MethodsAnATP-TCAwasusedtodeterminetheeffectof13singleorcombinedcytotoxicdrugsinsurgicalspecimensfrom114patientswithbreastcancercancer.ResultsTherewereconsiderabledifferencesinchemosensitivitybetweenindividuals.ThemostactivesingledrugintheassaywasidentifiedasPaclitaxelNavelbineEpirubicinMitomycinandadriamycin73.3%64.1%62.3%49.9%and40.0%ofspecimensshowedsensitivitytothemrespectively.Combinationofchemotherapy(Paclitaxel+EpirubicinCyclophosphamide+Epirubicin+5-Fluorouracil)wasfoundtobemoreeffectivethanrelativedrugsalone.ConclusionsTherewastheheterogeneityoftheinvitrochemosensitivityinbreastcancer.TheuseoftheATP-TCAprovideamethodofselectingappropriateanti-cancerdrugsinbreastcancer.[Keywords]ATP-tumorchemosensitivityassay;Breastcancer;Chemosensitivity