当归补血汤对兔动脉粥样硬化模型骨髓内皮祖细胞的影响.pdf

第34卷第9期2011年9月北京中医药大学学报Vol．34No．9Sep．2011JournalofBeIjIngUnIversItyofTradItIonalChIneseMedIcIne613当归补血汤对兔动脉粥样硬化模型骨髓内皮祖细胞的影响*秦臻黄水清#(广州中医药大学广东510405)摘要:目的观察当归补血汤对兔动脉粥样硬化(atherosclerosIs，As)模型骨髓内皮祖细胞(endothe-lIalprogenItorcells，EPCs)的影响，探讨当归补血汤防治As的可能作用机制。方法新西兰大白兔25只，免疫损伤联合高脂饮食法4周后形成As模型，模型动物按体重随机分为5组，每组5只。空白组灌胃等量蒸馏水，辛伐他汀组灌胃辛伐他汀水悬液1.7mg/kg，当归补血汤高、中、低剂量组分别灌胃当归补血汤6、3、1.5g/kg，每日1次，2周后取各组兔骨髓，密度梯度离心法分离单个核细胞，培养7d后将贴壁细胞与DIl标记的乙酰化低密度脂蛋白和FITC标记的荆豆凝集素1共同孵育鉴定EPCs。计算细胞集落形成数，MTT法检测增殖力，黏附实验检测黏附力，transwell小室检测迁移力，体外血管生成试剂盒检测成小管力。结果与空白组比较，当归补血汤高、中剂量组及辛伐他汀组骨髓EPCs集落数及增殖力、黏附力、迁移力、成小管力均提高(P＜0.05)，低剂量组则无明显变化。结论当归补血汤抗As的作用与促进EPCs数量和活性具有密切的关系。关键词:当归补血汤;内皮祖细胞;动脉粥样硬化;兔中图分类号:R285.5InfluenceofDanGGuiBuXueTanGonspinalendothelialproGenitorcellsinrabbitmodelofatherosclerosis*QINZhen，HUANGShuI-qIng#(GuangzhouUnIversItyofChIneseMedIcIne，Guangdong510405)Abstract:ObjectiveToobservetheInfluenceofDangGuIBuXueTangonspInalendothelIalprogenItorcells(EPCs)InrabbItmodelofatherosclerosIs，anddIscussthepossIblemechanIsmofDangGuIBuXueTangIntreatIngatherosclerosIs．MethodsThemodelofatherosclerosIswasestablIshedbyusIngImmuneInjuryandfattydIetfor4weeksInNewZealandrabbIts(n=25)．AllmodeledrabbItsweredIvIdedInto5groups(eachn=5)accordIngtothebodyweIght．TheblankgroupwasIntragastrIcallygIvendIstIlledwater，sImvastatIngroup，aqueoussuspensIonofsImvastatIn(1.7mg/kg)，andhIgh-dosegroup(6g/kg)，mId-dosegroup(3g/kg)andlow-dosegroup(1．5g/kg)，DangGuIBuXueTangonceadayfortwoweeks．ThemononuclearcellswereIsolatedfromrabbItbonemarrowbyusIngdensItygradIentcentrIfugatIon，whIchwereculturedfor7daysandthenadherentcellswereculturedwIthDIl-ac-LDLandFITC-UEA-1togetherforIdentIfyIngEPCsandcalculatIngthenumberofEPCscolonyformIng．TheprolIferatIonandadhesIonweredetectedbyusIngMTTmethod，adhensIon，byusIngadhensIondetermInatIon，mIgratIon，byusIngtranswellchambers，andangIogenesIs，byusIngInvItroangIogenesIskIt．ResultsComparedwIththeblankgroup，thenumberofEPCscolony，prolIfera-tIon，adhesIon，mIgratIonandangIogenes