电针对绝经后骨质疏松症模型大鼠肠黏膜钙结合蛋白-D9k.pdf

·944·ShanghaiJAcu-mox,Nov2013,Vol32,No11

文章编号:1005-0957(2013)11-0944-04·动物实验·
电针对绝经后骨质疏松症模型大鼠肠黏膜钙结合蛋白-D9k
mRNA和蛋白表达的影响

葛伟1,唐曦2,欧阳钢1,莫非1,侯立皓1,娄青林1,顾刘宝1
(1.江苏省省级机关医院,南京210024;2.重庆医科大学,重庆400016)

【摘要】目的观察电针对去卵巢大鼠模型肠黏膜钙结合蛋白-D9k(CaBP-D9K)mRNA和蛋白表达的影响,探讨针灸治疗绝经
后骨质疏松症可能机制。方法将60只3月龄SD健康雌性大鼠随机分为手术组和假手术组,3个月造模成功后将手术组随
机分为模型组、假电针组、电针组、药物组。干预治疗12星期后,测定腰椎及股骨骨密度(BMD),然后处死大鼠,提取小肠黏
膜,采用荧光实时定量PCR和Westernblotting检测CaBP-D9KmRNA和蛋白表达。结果成功复制绝经后骨质疏松症模型,
经电针组和药物组干预治疗12星期后,其大鼠骨密度与模型组相比均有明显提高,荧光实时定量PCR和Westernblotting
检测结果显示电针组和药物组大鼠小肠黏膜CaBP-D9KmRNA和蛋白表达上调,但电针组上调CaBP-D9KmRNA的水平不及药物
组。结论针灸治疗绝经后骨质疏松症机制之一可能与上调小肠黏膜CaBP-D9KmRNA和蛋白表达量,从而增强肠钙吸收有关。
【关键词】针刺疗法;电针;骨质疏松症,绝经后;钙结合蛋白-D9k;大鼠
【中图分类号】R2-03【文献标志码】A
DOI:10.3969/j.issn.1005-0957.2013.11.944
EffectofElectroacupunctureontheExpressionsofCaBP-D9KmRNAandProteininIntestinalMucosainaRatModelof
PostmenopausalOsteoporosisGEWei1,TANGXi2,OUYANGGang1,MOFei1,HOULi-hao1,LOUQing-lin1,
GULiu-bao1.1.JiangsuProvincialOrganizationHospital,Nanjing210024,China;2.ChongqingMedicalUniversity,Chongqing
400016,China
[Abstract]ObjectiveToinvestigatetheeffectofelectroacupunctureontheexpressionsofcalciumbindingprotein-D9k
(CaBP-D9K)mRNAandproteininintestinalmucosaofovariectomizedratsandexploreapossiblemechanismforacupuncture
treatmentofpostmenopausalosteoporosis.MethodsSixty3-month-oldhealthyfemaleSDratswererandomlyallocatedto
operationandshamoperationgroups.Followingsuccessfulmodelmakingafter3months,theoperationgroupwasrandomly
allocatedtomodel,shamelectroacupuncture,electroacupunctureandmedicationgroups.Lumbarvertebralandfemoralbonemineral
density(BMD)wasmeasuredafter12weeksofinterventionaltreatment.Theratswerethensacrificed.Smallintestinalmucosawas
taken.TheexpressionsofCaBP-D9KmRNAandproteinwereexaminedusingrealtimefluorescencequantitativePCRandWestern
blotting.ResultsAmodelofpostmenopausalosteoporosiswasmadesuccessfully.After12weeksofinterventionaltreatment,bone
mineraldensitywasincreasedsignificantlyintheelectroacupunctureandmedicationgroupscomparedwiththemodelgrou