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ABI73007500实时定量PCR仪相对定量实验入门指南.pdf

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入门指南 简介和相对定量 相对定量实验示例实验 美国应用生物系统公司7300/7500实时定量PCR仪 设计相对定量 实验 PrimerExtendedonmRNA 5′3′ 5′cDNA执行反转录 Reverse Primer Oligod(T)orrandomhexamer Synthesisof1stcDNAstrand 3′5′cDNA 从相对定量反应板 生成PCR数据 在相对定量研究 中生成PCR数据 ©Copyright2004,AppliedBiosystems.Allrightsreserved. ForResearchUseOnly.Notforuseindiagnosticprocedures. AuthorizedThermalCycler Thisinstrument,SerialNo___________,isanAuthorizedThermalCycler.Itspurchasepriceincludestheup-frontfee componentofalicenseunderUnitedStatesPatentNos.4,683,195,4,683,202and4,965,188,ownedbyRoche MolecularSystems,Inc.,andundercorrespondingclaimsinpatentsoutsidetheUnitedStates,ownedbyF.Hoffmann- LaRocheLtd,coveringthePolymeraseChainReaction("PCR")processtopracticethePCRprocessforinternal researchanddevelopmentusingthisinstrument.Therunningroyaltycomponentofthatlicensemaybepurchased fromAppliedBiosystemsorobtainedbypurchasingAuthorizedReagents.ThisinstrumentisalsoanAuthorized ThermalCyclerforusewithapplicationslicensesavailablefromAppliedBiosystems.ItsusewithAuthorized ReagentsalsoprovidesalimitedPCRlicenseinaccordancewiththelabelrightsaccompanyingsuchreagents. PurchaseofthisproductdoesnotitselfconveytothepurchaseracompletelicenseorrighttoperformthePCR process.FurtherinformationonpurchasinglicensestopracticethePCRprocessmaybeobtainedbycontactingthe DirectorofLicensingatAppliedBiosystems,850LincolnCentreDrive,FosterCity,California94404. DISCLAIMEROFLICENSE:Norightsforanyapplication,includinganyinvitrodiagnosticapplication,are conveyedexpressly,byimplicationorbyestoppelunderanypatentorpatentapplicationsclaiminghomogeneousor real-timedetectionmethods,includingpatentscoveringsuchmethodsusedinconjunctionwiththePCRprocessor otheramplificationprocesses.The5'nucleasedetectionassayandcertainotherhomogeneousorreal-time amplificationanddetectionmethodsarecoveredbyUnitedStatesPatentNos.5,210,015,5,487,972,5,804,375and 5,994,056,ownedbyRocheMolecularSystems,Inc.;bycorrespondingpatentsandpatentapplicationsoutsidethe UnitedStates,ownedbyF.Hoffma