纳米银抗菌活性和细胞毒性评价.docx

纳米银抗菌活性和细胞毒性评价纳米银抗菌活性和细胞毒性评价摘要：以大肠杆菌和金黄色葡萄球菌做模型检测抗菌活性,选择L-929细胞通过MTT法评价毒性.结果证明纳米银具有显著的抗菌活性且呈剂量依赖性;不论在液体培养基还是固体培养基,30×10浓度产生的抗菌作用大于50%,但有潜在的细胞毒性;即使在3×10浓度下.细胞毒性达到2级,有26%,～50%的细胞生长受到抑制.Abstract：E.coilandS.aureuswereusedasmodelfortestingantimicrobialactivity,andL-929celllinewasselectedfortoxicityevaluationbycellularmitochondrialfunction(MTTassay).Theresultsshowthatsilvernanoparticles(AgNPs)cansignificantlyin-creaseantibacterialactivitywiththeincreasingofconcentrationofthesilvernanoparticles.Thelowesteffectiveconcentrationofsilvernanoparticles(30X10)whichleadstomorethan50%ofinhibitioninliquidmediumandagarplateonbacteriasisstillsatis-factorybutwillproducepotentialcytotoxicity.Evenat3x10concentrationofsilvernanoparticlesthecytotoxicitystillexistswith2gradewhichrepresents26%-50%ofcellgrowthinhibited,and30×10displayssimilarimpact.作者：李新平[1]李胜利[2]张淼涛[1]张文龙[1]李创宏[1]Author：LiXinping[1]  LiShengli[2]  ZhangMiaotao[1]  ZhangWenlong[1]  LiChuanghong[1]作者单位：西北农林科技大学,陕西,杨凌,712100中国农业大学动物营养国家重点实验室,北京,100083期刊：稀有金属材料与工程 ISTICEISCIPKUJournal：RAREMETALMATERIALSANDENGINEERING年，卷(期)：2011, 40(2)分类号：Q2关键词：纳米银  大肠杆菌  金黄色葡萄球菌  抗菌活性  细胞毒性  Keywords：silvernanoparticles  S.aureus  E.coli  antibacterialactivity  cytotoxicity  机标分类号：O6P61机标关键词：纳米银    抗菌活性    细胞毒性评价    AgNanoparticles    silvernanoparticles    antimicrobialactivity    antibacterialactivity    toxicityevaluation    金黄色葡萄球菌    cellgrowth    液体培养基    剂量依赖性    固体培养基    L-929细胞    effective    cellline    细胞生长    浓度    模型检测  &