水稻白叶枯病菌生理小种在纳米银膜上的表面增强拉曼光谱初探.docx

水稻白叶枯病菌生理小种在纳米银膜上的表面增强拉曼光谱初探水稻白叶枯病菌生理小种在纳米银膜上的表面增强拉曼光谱初探摘要：用硝酸银和聚乙烯醇混合溶液作为电解液,把载玻片放入电解液中,用银棒作为电极在直流电压下电解1h,使载玻片在电解出的银胶中静置12h后,取小晾干,即得制备的银膜.用便携式拉曼光谱仪测得在银膜上的白叶枯病菌7个生理小种的表而增强拉曼光谱(SERS).7个生理小种分别是1-YN1,2-YN7,3-YN11,4-GD414,5-SCYC6,6-HEN11,7-FWJ,7个生理小种的SERS谱在峰位及峰的相对强度上都有很多差别.这可用于生理小种的鉴别.因为银膜上的纳米银颗粒是银胶中银颗粒的.聚合体,这种聚合体大大缩小了纳米银之间的距离,从而产生了很强的电磁增强作用.电解法制备的银膜价格低廉,增强效果好,普通实验室便可以制备.此研究为快速、方便检测白叶枯病菌不间生理小种提出了一种新的方法,对白叶枯病的防治具有重要的意义.Abstract：Thenanosilverfilmwaspreparedbyelectrolysismethodusingsilvernitrateandpolyvinylalcohol(PVA)indeionizedwaterastheelectrolyte,withfourglassslidesputintheelectrolyteandtwosilverrodsdippedintotheelectrolyteastheanodeandcathode.Adirectcurrentwasappliedtotherods,thenthefourglassslidesstayedinthesilvercolloids.Thustheauthorsgotthenanosilverfilm.Transmissionelectronmicroscopy(TEM)andscanningelectronmicroscopy(SEM)wereemployedtodetectthesilverparticlesinthesilvercolloidsandonthenanosilverfilm.FromtheSEMwecanseethatthesilverparticlesonthefilmformeddifferentlayers.Inonelayer,thedistancebetweentwoparticleswasabout100nm.ThesamplesofXanthomonasoryzaepv.Oryzae(Xoo)were7differentkindsofbacterialblight,namely1-YN1,2-YN7,3-YN11,4-GD414,5SCYC6,6-HEN11and7-FWJ.Becausethesilverparticlesinthecolloidswereaggregatedonthefilm,therewaslargeelectromagneticpotentiation.SotheSERSspectraofXoowereperfect.TheauthorsusedtheareaanalyticalmethodtodistinguishthedifferentkindsofXoo.Thesilverfilmpreparedbyelectrolysiswascheapandactive,thepreparationtimeofthesampleswasshort,andanynormalchemistrylabcanmakeit,whichcanfindexcellentapplicationtodetectingtheXooinagriculture.Ontheotherhand,thisfilmisactiveonbiomoleculesandbioorganisrn,whichmaybeanewkindofSERSfundustoexplainthecreationoftheSERS.Furtherstudywasunderway.作者：康颐璞[1]司民真[1]李清玉[2]黄琼[3]刘仁明[1]Author：KANGYi-pu[1]  SIMin-zhen[1]  LIQing-yu[2]  HUANGQiong[3]  LIURen-ming[1]作者单位：楚雄师范学院物理与电子科学系,云南,楚雄,675000西南林学院基础部,云南,昆明,650224云南农业大学植保学院,云南,昆明,650201期刊：光谱学与光谱分析 ISTICEISCIPKUJournal：SPECTROSCOPYAND