针刺对抑郁症模型大鼠海马区单胺类神经递质的影响.docx

针刺对抑郁症模型大鼠海马区单胺类神经递质的影响针刺对抑郁症模型大鼠海马区单胺类神经递质的影响【摘要】目的观察针刺对抑郁症模型大鼠的作用机制。方法选择Open-Field法评分相近的40只成年SD雄性大鼠随机分为正常对照组、模型组、药物组、针刺组，每组各10只。应用分养和长期不可预见性中等强度应激造成抑郁症模型大鼠。以Open-Field法对照研究针刺对慢性应激诱导的抑郁症模型大鼠行为学的改变；应用高效液相电化学检测仪检测单胺类神经递质。结果针刺组大鼠，在治疗第7天的行为学评分与正常对照组之间已经无明显差异（P＞0.05）；在治疗21天时，与药物氟西汀组行为学评分无明显差异（P＞0.05），与模型对照组大鼠差异存在显著性（P＜0.01）。而在治疗21天后，针刺组大鼠海马内5-HT和NE的含量，与正常组差异无显著性（P＞0.05）；与模型对照组比较，差异存在显著性（P＜0.05，P＜0.01）。结论针刺治疗抑郁症模型大鼠的行为学异常，其疗效与氟西汀相当；抑郁症模型大鼠海马区5-HT和NE的含量与Open-field评分存在相似性；针刺疗效与海马区单胺类神经递质的改变有关系。【关键词】针刺治疗；抑郁症；疾病模型；大鼠；行为学；5-HT；NEEffectsofacupunctureonserotoninandnorepinephrineinhippocampusofdepressedmodelrats【Abstract】ObjectiveToobservehowacupunctureaffectsthemechanismofdepressionmodelrats.MethodsUsingOpen-Fieldmethodtorandomlyselect40adultSDmaleratsintonormalcontrol,modelgroup,drugtreatmentandacupuncturetreatmentgroup,10ratspergroup.Separation,longtermunpredictability,mediumstimulationtechniqueswereappliedtomodifyrats?behavior.ThenusingOpen-Fieldmethodtoreflectchangesindepressedmodelrats?behaviorbetweengroupstreatedwithacupunctureandcontrol.AndtheconcentrationofmonoamineneurotransmittersandtheirmetabolitesinhippocampusofratbrainweredeterminedbyHPLC-ECD.ResultsComparedwithnormalcontrol,acupuncturetreatmentgroup’sbehaviorwasnotdifferent（P＞0.05）onthe7thdayaftertreatment.Onthe21thdayaftertreatment,acupuncturetreatmentgroup’sbehaviorwasnotdifferent（P＞0.05）withdrugtreatmentgroup,andwasmoredifferentwithmodelgroup（P＜0.01）.Comparedwithnormalgrouponthe21thdayoftreatment,theconcentrationofserotoninandnorepinephrineinhippocampusofacupuncturetreatmentgroupwasnotdifferent（P＞0.05）,whereaswasmoredifferentwithmodelgroup（P＜0.05，P＜0.01）.ConclusionThisexperimentprovedthatacupuncturetreatmentcouldchangethebehaviorabnormalityofdepressivemodelrats.Thecurativeeffectsiscomparabletoflouxetine.Thechangesoftheconcentrationofserotoninandnorepinephrineinhippocampusareoneofthemechanism.【Keywords】acupuncturetreatment；depression；diseasemodel；rat；behavior；5hydroxytryptamine(5-HT);NE自1984年，针灸开始介入抑郁症的治疗以来，取得了确