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SterilizationofEscherichiacolice.docx

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SterilizationofEscherichiacoliceSterilizationofEscherichiacolicellsbytheapplicationofpulsedmagneticfieldAbstract：Theinactivationofmicroorganismsbypulsedmagneticfieldwasstudied.ItwasimprovedthattheapplicationofelectromagneticpulsesevidentlycausesalethaleffectonE.colicellssuspendedinphosphatebuffersolutionNa2HPO4/NaH2PO4(0.334/0.867mmol/L).Experimentalresultsindicatedthatthesurvivability(N/N0;whereN0andNarethenumberofcellssurvivedpermilliliterbeforeandafterelectromagneticpulsesapplication,respectively)ofE.colidecreasedwithmagneticfieldintensityBandtreatmenttimet.Itwasalsofoundthatthemediumtemperatures,thefrequenciesofpulsef,andtheinitialbacterialcellconcentrationshavedeterminateinfluencesindestructionofE.colicellsbytheapplicationofmagneticpulses.TheapplicationofanmagneticintensityB=160mTatpulsesfrequencyf=62kHzandtreatmenttimet=16hresultinaconsiderabledestructionlevelsofE.colicells(N/N0=10-4).Possiblemechanismsinvolvedinsterilizationofthemagneticfieldtreatmentwerediscussed.Inordertoshortenthetreatmenttime,manygroupsofparallelinductivecoilwereused.Thepracticabilitytestshowedthatthetreatmenttimewasshortenedto4hwiththeapplicationofthreegroupsofparallelcoilwhenthesurvivabilityofE.colicellswaslessthan0.01%;andthepowerconsumptionwasabout0.2kWh/m3.作者：LIMei  QUJiu-hui  PENGYong-zhen 作者单位：LIMei(ResearchCenterforEco-EnvironmentalSciences,ChineseAcademyofSciences,Beijing100085,China;SchoolofMunicipalandEnvironmentalEngineering,HarbinInstituteofTechnology,Harbin150090,China)QUJiu-hui(ResearchCenterforEco-EnvironmentalSciences,ChineseAcademyofSciences,Beijing100085,China)PENGYong-zhen(SchoolofMunicipalandEnvironmentalEngineering,HarbinInstituteofTechnology,Harbin150090,China) 期刊：环境科学学报(英文版) ISTICSCI Journal：JOURNALOFENVIRONMENTALSCIENCES 年，卷(期)：2004, 16(2) 分类号：X703 Keywords：Escherichiacolibacteria  pulsedmagneticfield  inducedcurrent  cellmembrane